Antioxidant and Anticoagulant Activity of Microbial Nano Cellulose-ZnO-Ag Composite Components.

<b>Background and Objectives:</b> Bacterial cellulose (BC) is a microbial extracellular biopolymer formed by microbial strains like <i>Gluconacetobacter xylinus</i>. The objective of this study was to determine the antioxidant and anticoagulant of a microbial nano cellulose-ZnO-Ag (CNCs) composite and its components separately. <b>Materials and Methods:</b> Three components were used for nano cellulose-ZnO-Ag composite synthesis, Ag-nanoparticles, ZnO-nanoparticles and BC. The DPPH method was used to calculate the scavenging of free radical behaviour of four different composite samples. <b>Results:</b> Results of silver nanoparticles were found to have the highest antioxidant activity with IC₅₀ 65 μg mL¹, followed by CNCs-ZnO-Ag composite (IC₅₀ 88.98 μg mL¹) but ZnONPs IC₅₀ was 263 μg mL¹ and BC (IC₅₀ 955 μg mL¹). The CNCs-ZnO-Ag composite, BC and AgNPs at 25 μg mL¹ had clotting times that were nearly identical to the control. The APTT increased to 56 Sec at 75 μg mL¹ of CNCs-ZnO-Ag composite related to control that recorded 33 Sec. <b>Conclusion:</b> Bacterial cellulose acquired new activity in nano form and also when conjugated with nanoparticles. The CNCs-ZnO-Ag composite is ready for pharmaceutical application as an antioxidant and anticoagulant after <i>in vivo</i> study.