Lecturer of Microbiology, Faculty of Applied Health Sciences Technology, October 6 University, Giza Governorate, Egypt.
Assistant professor of Microbiology, Faculty of Applied Health Sciences Technology, October 6 University, Giza Governorate, Egypt.
Microb Cell Fact. 2024 Mar 27;23(1):92. doi: 10.1186/s12934-024-02360-x.
Excessive consumption of antibiotics is considered one of the top public health threats, this necessitates the development of new compounds that can hamper the spread of infections. A facile green technology for the biosynthesis of Zinc oxide nanoparticles (ZnO NPs) using the methanol extract of Spirulina platensis as a reducing and stabilizing agent has been developed. A bunch of spectroscopic and microscopic investigations confirmed the biogenic generation of nano-scaled ZnO with a mean size of 19.103 ± 5.66 nm. The prepared ZnO NPs were scrutinized for their antibacterial and antibiofilm potentiality, the inhibition zone diameters ranged from 12.57 ± 0.006 mm to 17.33 ± 0.006 mm (at 20 µg/mL) for a variety of Gram-positive and Gram-negative pathogens, also significant eradication of the biofilms formed by Staphylococcus aureus and Klebsiella pneumoniae by 96.7% and 94.8% respectively was detected. The free radical scavenging test showed a promising antioxidant capacity of the biogenic ZnO NPs (IC78.35 µg/mL). Furthermore, the anti-inflammatory role detected using the HRBCs-MSM technique revealed an efficient stabilization of red blood cells in a concentration-dependent manner. In addition, the biogenic ZnO NPs have significant anticoagulant and antitumor activities as well as minimal cytotoxicity against Vero cells. Thus, this study offered green ZnO NPs that can act as a secure substitute for synthetic antimicrobials and could be applied in numerous biomedical applications.
抗生素的过度使用被认为是对公共健康的最大威胁之一，因此需要开发新的化合物来阻止感染的传播。本研究采用螺旋藻甲醇提取物作为还原剂和稳定剂，开发了一种简便的绿色技术，用于生物合成氧化锌纳米粒子（ZnO NPs）。一系列光谱和显微镜研究证实了纳米级 ZnO 的生物生成，其平均尺寸为 19.103 ± 5.66nm。研究了制备的 ZnO NPs 的抗菌和抗生物膜潜力，抑菌圈直径范围为 12.57 ± 0.006mm 至 17.33 ± 0.006mm（在 20μg/mL 时），针对多种革兰氏阳性和革兰氏阴性病原体，还分别检测到对金黄色葡萄球菌和肺炎克雷伯菌形成的生物膜的显著清除率为 96.7%和 94.8%。自由基清除试验显示生物合成的 ZnO NPs 具有良好的抗氧化能力（IC78.35μg/mL）。此外，使用 HRBCs-MSM 技术检测到的抗炎作用显示出红细胞在浓度依赖性方式下的有效稳定作用。此外，生物合成的 ZnO NPs 具有显著的抗凝和抗肿瘤活性，对 Vero 细胞的细胞毒性最小。因此，本研究提供了绿色 ZnO NPs，可作为合成抗菌剂的安全替代品，并可应用于许多生物医学应用。
