Mizumoto T
Hokkaido Igaku Zasshi. 1987 Mar;62(2):332-45.
The present study was undertaken to elucidate the effect of calcium ion on the granulation tissue formation during wound healing. Male guinea pigs of Pilbright strain were chosen as the experimental animal. An appropriate open wound was aseptically made on the dorsum of the guinea pigs, which was then equipped with a sterile Teflon-cylinder. The amounts of granulation tissues developed within the cylinder were determined during 10 day period of the observation. In addition, various concentrations of CaCl2 were applied on the wound surface for up to 3 days after the wounding. Calmodulin contents in the granulation tissue were also determined using calmodulin-deficient phosphodiesterase prepared from bovine heart. Purified pig skin epidermal calmodulin was used as the standard. The results obtained were as follows: The amount of the granulation tissue increased in a stepwise manner during 3, 5, 7, and 10 days after the wounding. Calmodulin content in the granulation tissue was maximal on the day 3 after the wounding, waned on the day 5, and remained low on the days 7 and 10. A significant augmentation of the granulation tissue production as well as of calmodulin content was observed when CaCl2 was applied on the wound surface during 3 days after the wounding. These results suggest that calcium ion might have a significant role on granulation tissue development, possibly through the Ca++-calmodulin system, during early stages of wound healing process.
本研究旨在阐明钙离子在伤口愈合过程中对肉芽组织形成的影响。选用皮尔布赖特品系的雄性豚鼠作为实验动物。在豚鼠背部无菌制造一个合适的开放性伤口，然后安装一个无菌的聚四氟乙烯圆柱体。在观察的10天期间内测定圆柱体内形成的肉芽组织量。此外，在受伤后3天内，将不同浓度的CaCl2应用于伤口表面。还使用从牛心制备的钙调蛋白缺陷型磷酸二酯酶测定肉芽组织中的钙调蛋白含量。以纯化的猪皮肤表皮钙调蛋白作为标准品。获得的结果如下：受伤后3、5、7和10天，肉芽组织量呈逐步增加。肉芽组织中的钙调蛋白含量在受伤后第3天最高，在第5天下降，在第7天和第10天保持较低水平。当在受伤后3天内将CaCl2应用于伤口表面时，观察到肉芽组织生成以及钙调蛋白含量显著增加。这些结果表明，在伤口愈合过程的早期阶段，钙离子可能通过Ca++-钙调蛋白系统对肉芽组织发育起重要作用。
