Lansdown A B, Sampson B, Laupattarakasem P, Vuttivirojana A
Department of Comparative Biology, Charing Cross and Westminster Medical School, London, U.K.
Br J Dermatol. 1997 Nov;137(5):728-35.
Incisional wounds 15 mm long were induced surgically in the back skin of young adult Wistar rats. They were sutured and used as an experimental model in the therapeutic evaluation of daily applications of 0.5 mL of silver nitrate (SN) at 0.01, 0.1 or 1.0% w/v aqueous solution, or 0.5 g silver sulphadiazine (SSD) over a 10-day period. Control wounds received deionized water only. The silver preparations were not toxic but SN did stain the hair and superficial layers of the stratum corneum. The wounds remained microbiologically clean. Wounds exposed to SN (0.1 or 1.0%) or SSD healed more rapidly than controls. From about the fourth day of treatment, we noted a more rapid exteriorization of sutures, improved wound closure and an earlier loss of scabs and wound debris. Silver treatment appeared to reduce the inflammatory and granulation tissue phases of healing and enhance epidermal repair. Silver from SN was deposited as silver sulphide in extrafollicular hair shafts and superficial aspects of the skin and wound debris but not at deeper levels. Silver uptake was four-fold higher in damaged skin than in intact tissue. SSD was absorbed by intact and wounded skin but the silver did not precipitate as silver sulphide and its localization in the tissue is not known. Uptake of silver from SN or SSD was associated with changes in the concentrations of zinc and calcium in the skin. Zinc levels were depressed during the inflammatory and proliferative phases of healing and then increased. Zinc concentrations had normalized by 10 days when wound healing was achieved. Calcium levels remained higher than normal throughout the observation period. The mechanism of action of silver in advancing wound healing in the rat is unclear. Its ability to reduce the inflammatory and granulation phases of healing, and to invoke metallothionein production and influence metal ion binding are possibly important.
在成年Wistar幼鼠背部皮肤进行手术制造15毫米长的切口伤口。将伤口缝合，作为实验模型，用于评估在10天时间里每日涂抹0.5毫升0.01%、0.1%或1.0%（重量/体积）硝酸银（SN）水溶液，或0.5克磺胺嘧啶银（SSD）的治疗效果。对照伤口仅接受去离子水。银制剂无毒，但SN确实会使毛发和角质层表层染色。伤口在微生物学上保持清洁。暴露于SN（0.1%或1.0%）或SSD的伤口比对照伤口愈合更快。从治疗大约第四天起，我们注意到缝线更快外露、伤口闭合改善以及痂皮和伤口碎屑更早脱落。银处理似乎减少了愈合的炎症和肉芽组织阶段，并增强了表皮修复。来自SN的银以硫化银的形式沉积在毛囊外毛干以及皮肤和伤口碎屑的表层，但未沉积在更深层。受损皮肤对银的摄取比完整组织高四倍。SSD被完整皮肤和受伤皮肤吸收，但银不会沉淀为硫化银，其在组织中的定位尚不清楚。从SN或SSD摄取银与皮肤中锌和钙浓度的变化有关。锌水平在愈合的炎症和增殖阶段降低，然后升高。到伤口愈合的第10天，锌浓度恢复正常。在整个观察期内，钙水平一直高于正常。银促进大鼠伤口愈合的作用机制尚不清楚。其减少愈合的炎症和肉芽阶段、引发金属硫蛋白产生以及影响金属离子结合的能力可能很重要。
