与宫颈发育不良女性血浆中可溶性碳酸酐酶 IX 浓度升高相关的因素。

Factors, associated with elevated concentration of soluble carbonic anhydrase IX in plasma of women with cervical dysplasia.

机构信息

Department of Biothermodynamics and Drug Design, Institute of Biotechnology, Life Sciences Center, Vilnius University, Sauletekio sl. 7, 10257, Vilnius, Lithuania.

Department of Obstetrics and Gynecology, Medical Academy, Lithuanian University of Health Sciences, Eiveniu st. 2, 50161, Kaunas, Lithuania.

出版信息

Sci Rep. 2022 Sep 13;12(1):15397. doi: 10.1038/s41598-022-19492-y.

DOI:10.1038/s41598-022-19492-y

PMID:36100684

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9470728/

Abstract

UNLABELLED

Precancerous lesions of human cervix uteri have a tendency for regression or progression. In cervical intraepithelial neoplasia grade 2 (CINII) case there is an uncertainty if a lesion will progress or regress. The carbonic anhydrase IX (CAIX) enzyme is overexpressed in cervical cancer which is more sensitive to radiotherapy. CAIX is associated with poor prognosis in solid hypoxic tumors. The aim of this study was to determine factors related to elevated soluble CAIX (s-CAIX) in high-grade intraepithelial lesion (HSIL) cases.

METHODS

Patients diagnosed with HSIL (N = 77) were included into the research group whereas without HSIL (N = 72)-the control group. Concentration of the soluble CAIX (s-CAIX) in plasma was determined by the DIANA ligand-antibody-based method. C. trachomatis was detected from cervical samples by PCR. Primary outcomes were risk factors elevating s-CAIX level in HSIL group. Non-parametric statistical analysis methods were used to calculate correlations.

RESULTS

The s-CAIX level in patients with HSIL was elevated among older participants (r = 0.27, p = 0.04) and with C. trachomatis infection (p = 0.028). Among heavy smokers with HSIL, the concentration of s-CAIX was higher in older women (r = 0.52, p = 0.005), but was not related to the age of heavy smokers' controls (τ = 0.18 p = 0.40).

CONCLUSION

The concentration of s-CAIX was higher among older, heavy smoking and diagnosed with C. trachomatis patients. All these factors increased the risk for HSIL progression.

摘要

未注明

人宫颈的癌前病变有消退或进展的趋势。在宫颈上皮内瘤变 2 级（CINII）病例中，病变是否会进展或消退存在不确定性。碳酸酐酶 IX（CAIX）在宫颈癌中过度表达，对放疗更敏感。CAIX 与实体缺氧肿瘤的不良预后相关。本研究旨在确定与高级上皮内病变（HSIL）病例中可溶性 CAIX（s-CAIX）升高相关的因素。

方法

将诊断为 HSIL（N=77）的患者纳入研究组，而无 HSIL（N=72）的患者纳入对照组。采用 DIANA 配体-抗体为基础的方法测定血浆中可溶性 CAIX（s-CAIX）的浓度。采用 PCR 从宫颈标本中检测沙眼衣原体。主要结局是确定 HSIL 组中升高 s-CAIX 水平的危险因素。采用非参数统计分析方法计算相关性。

结果

HSIL 患者的 s-CAIX 水平在年龄较大的患者（r=0.27，p=0.04）和感染沙眼衣原体的患者（p=0.028）中升高。在患有 HSIL 的重度吸烟者中，s-CAIX 浓度在年龄较大的女性中较高（r=0.52，p=0.005），但与重度吸烟者对照组的年龄无关（τ=0.18，p=0.40）。