Bartkiene Elena, Starkute Vytaute, Zokaityte Egle, Klupsaite Dovile, Mockus Ernestas, Bartkevics Vadims, Borisova Anastasija, Gruzauskas Romas, Liatukas Žilvinas, Ruzgas Vytautas
Institute of Animal Rearing Technologies, Lithuanian University of Health Sciences, Tilzes Str. 18, LT-47181 Kaunas, Lithuania.
Department of Food Safety and Quality, Lithuanian University of Health Sciences, Tilzes G. 18, LT-47181 Kaunas, Lithuania.
Biology (Basel). 2022 Jun 27;11(7):966. doi: 10.3390/biology11070966.
The aim of this study was to analyze and compare the acidity, microbiological, and chromaticity parameters; fatty acid (FA) and volatile compound (VC) profiles; and biogenic amine (BA), macro- and microelement, and mycotoxin concentrations in nontreated ‘Ada’, ‘Sarta’, and new breed blue (DS8472-5) and purple (DS8526-2) wheat lines wholemeal (WW) with those fermented with lactic acid bacteria (LAB) possessing antimicrobial/antifungal properties, isolated from spontaneous sourdough: Pediococcus acidilactici-LUHS29, Liquorilactobacillus uvarum-LUHS245, Lactiplantibacillus plantarum-LUHS122). All the fermented WW showed >8.0 log10 CFU/g of LAB count, and the type of LAB was a significant factor in the WW acidity parameters. Phenylethylamine was the predominant BA in WW, and the wheat variety (WV), the type of LAB, and their interaction were significant factors on the BA formation. Despite the fact that some differences in trace element concentrations in WW were obtained, in most of the cases fermentation was not a significant factor in their content. The main FAs in WW were palmitic acid, all-cis,trans-octadecenoic acid, and linoleic acid. Fermented WW showed a more diverse VC profile; however, the influence of fermentation on deoxynivalenol in WW was varied. Finally, further studies are needed to indicate the technological parameters that would be the most effective for each WV, including the lowest BA formation and mycotoxin degradation.
本研究的目的是分析和比较未经处理的‘阿达’、‘萨尔塔’以及新品种蓝色(DS8472 - 5)和紫色(DS8526 - 2)小麦品系全麦粉(WW)与用从自发酸面团中分离出的具有抗菌/抗真菌特性的乳酸菌(LAB)发酵后的全麦粉的酸度、微生物和色度参数;脂肪酸(FA)和挥发性化合物(VC)谱;以及生物胺(BA)、常量和微量元素和霉菌毒素浓度(植物乳杆菌 - LUHS122、嗜酸乳杆菌 - LUHS29、葡萄汁乳杆菌 - LUHS245)。所有发酵后的全麦粉LAB计数均>8.0 log10 CFU/g,LAB类型是影响全麦粉酸度参数的重要因素。苯乙胺是全麦粉中主要的生物胺,小麦品种(WV)、LAB类型及其相互作用是影响生物胺形成的重要因素。尽管在全麦粉中微量元素浓度存在一些差异,但在大多数情况下,发酵并非影响其含量的重要因素。全麦粉中的主要脂肪酸为棕榈酸、全顺式、反式十八碳烯酸和亚油酸。发酵后的全麦粉VC谱更为多样;然而,发酵对全麦粉中脱氧雪腐镰刀菌烯醇的影响各不相同。最后,需要进一步研究以确定对每个小麦品种最有效的工艺参数,包括最低的生物胺形成和霉菌毒素降解。