Luna-García Luis A, Meza-Herrera César A, Pérez-Marín Carlos C, Corona Rebeca, Luna-Orozco Juan R, Véliz-Deras Francisco G, Delgado-Gonzalez Ramón, Rodriguez-Venegas Rafael, Rosales-Nieto Cesar A, Bustamante-Andrade Jorge A, Gutierrez-Guzman Ulises N
Universidad Autónoma Chapingo, Unidad Regional Universitaria de Zonas Áridas, Bermejillo 35230, Durango, Mexico.
Departamento de Medicina y Cirugía Animal, Campus Rabanales, Universidad de Córdoba, 14014 Córdoba, Spain.
Biology (Basel). 2022 Jul 6;11(7):1015. doi: 10.3390/biology11071015.
The potential effect of intravenous administration of glutamate on the ovarian activity and the LH secretion pattern, considering the anestrous yearling goat as an animal model, were assessed. In late April, yearling goats (n = 20) were randomly assigned to either (1) Glutamate supplemented (GLUT; n = 10, Live Weight (LW) = 29.6 ± 1.02 kg, Body Condition (BCS) = 3.4 ± 0.2 units; i.v. supplemented with 7 mg GLUT kg−1 LW) or (2) Non-supplemented (CONT; n = 10; LW = 29.2 ± 1.07 kg, BCS = 3.5 ± 0.2 units; i.v. saline). The oats were estrus-synchronized; blood sampling (6 h × 15 min) was carried out for LH quantification. Response variables included pulsatility (PULSE), time to first pulse (TTFP), amplitude (AMPL), nadir (NAD), and area under the curve (AUC) of LH. Ovaries were ultra-sonographically scanned to assess ovulation rate (OR), number of antral follicles (AF), and total ovarian activity (TOA = OR + AF). LH-PULSE was quantified with the Munro algorithm; significant treatment x time interactions were evaluated across time. The variables LW and BCS did not differ (p > 0.05) between the experimental groups. Nevertheless, OR (1.77 vs. 0.87 ± 0.20 units), TOA (4.11 vs. 1.87 ± 0.47 units) and LH-PULSE (5.0 vs. 2.2 pulses 6 h-1) favored (p < 0.05) to the GLUT group. Our results reveal that targeted glutamate supplementation, the main central nervous system neurotransmitter, arose as an interesting strategy to enhance the hypothalamic−hypophyseal−ovarian response considering the anestrous-yearling goat as an animal model, with thought-provoking while promising translational applications.
以处于乏情期的一岁龄山羊作为动物模型，评估了静脉注射谷氨酸对卵巢活性和促黄体生成素（LH）分泌模式的潜在影响。4月下旬，将一岁龄山羊（n = 20）随机分为两组：（1）谷氨酸补充组（GLUT；n = 10，体重（LW）= 29.6 ± 1.02 kg，体况评分（BCS）= 3.4 ± 0.2单位；静脉注射补充7 mg GLUT·kg−1体重）或（2）非补充组（CONT；n = 10；LW = 29.2 ± 1.07 kg，BCS = 3.5 ± 0.2单位；静脉注射生理盐水）。对山羊进行发情同步处理；进行血液采样（6小时×15分钟）以定量LH。反应变量包括LH的脉冲性（PULSE）、首次脉冲时间（TTFP）、幅度（AMPL）、最低点（NAD）和曲线下面积（AUC）。对卵巢进行超声扫描以评估排卵率（OR）、窦状卵泡数量（AF）和总卵巢活性（TOA = OR + AF）。用Munro算法对LH脉冲进行定量；评估不同时间点显著的处理×时间交互作用。实验组之间的LW和BCS变量没有差异（p > 0.05）。然而，OR（1.77对0.87 ± 0.20单位）、TOA（4.11对1.87 ± 0.47单位）和LH脉冲（5.0对2.2次脉冲·6小时−1）有利于（p < 0.05）GLUT组。我们的结果表明，以处于乏情期的一岁龄山羊作为动物模型，靶向补充谷氨酸（主要的中枢神经系统神经递质）是一种增强下丘脑-垂体-卵巢反应的有趣策略，具有引人深思且有前景的转化应用价值。
