Lopez-Flores Noé M, Meza-Herrera César A, Perez-Marin Carlos, Blache Dominique, Arellano-Rodríguez Gerardo, Zuñiga-Garcia Santiago, Navarrete-Molina Cayetano, Peña Cristina García De la, Rosales-Nieto Cesar A, Veliz-Deras Francisco G
Regional Universitary Unit on Arid Lands-URUZA, Chapingo Autonomous University, Bermejillo, Durango 35230, Mexico.
Institute for Graduate Studies-IDEP, University of Cordoba, 14014 Córdoba, Spain.
Animals (Basel). 2020 Apr 10;10(4):659. doi: 10.3390/ani10040659.
The possible out-of-season effect of beta-carotene supplementation on ovulation rate (OR), antral follicles (AFN), and total ovarian activity (TOA = OR + AFN) as related to the LH release pattern in yearling anestrous goats was evaluated. In late April, Alpine-Saanen-Nubian x Criollo goats ( = 22, 26 N) were randomly allotted to: (1) Beta-carotene (BETA; = 10, orally supplemented with 50 mg/goat/d; 36.4 ± 1.07 kg live weight (LW), 3.5 ± 0.20 units, body condition score (BCS) or (2) Non-supplemented (CONT; = 12, 35.2 ± 1.07 kg LW, 3.4 ± 0.2 units BCS). Upon estrus synchronization, an intensive blood sampling (6 h × 15 min) was accomplished in May for LH quantifications; response variables included (pulsatility-PULSE, time to first pulse-TTFP, amplitude-AMPL, nadir-NAD and area under the curve-AUC). Thereafter, an ultrasonography scanning was completed to assess OR and AFN. The Munro algorithm was used to quantify LH pulsatility; if significant effects of time, treatment or interaction were identified, data were compared across time. Neither LW nor BCS ( > 0.05) or even the LH ( > 0.05); PULSE (4.1 ± 0.9 pulses/6 h), NAD (0.47 ± 0.13 ng) and AUC (51.7 ± 18.6 units) differed between treatments. Nonetheless, OR (1.57 vs. 0.87 ± 0.18 units) and TOA (3.44 vs. 1.87 ± 0.45 units) escorted by a reduced TTFP (33 vs. 126 ± 31.9 min) and an increased AMPL (0.55 vs. 0.24 ± 0.9 ng), favored to the BETA supplemented group ( < 0.05), possibly through a GnRH-LH enhanced pathway and(or) a direct effect at ovarian level. Results are relevant to speed-up the out-of-season reproductive outcomes in goats while may embrace translational applications.
评估了补充β-胡萝卜素对一岁发情期山羊排卵率（OR）、窦卵泡（AFN）和总卵巢活性（TOA = OR + AFN）的可能的非季节性影响，这些影响与促黄体生成素（LH）释放模式有关。4月下旬，将阿尔卑斯-萨能-努比亚×克里奥罗山羊（n = 22，26只）随机分为：（1）β-胡萝卜素组（BETA；n = 10，口服补充50 mg/只/天；活重（LW）36.4 ± 1.07 kg，体况评分（BCS）3.5 ± 0.20分）或（2）非补充组（CONT；n = 12，LW 35.2 ± 1.07 kg，BCS 3.4 ± 0.2分）。在发情同步后，于5月进行密集采血（6小时×15分钟）以测定LH含量；反应变量包括（脉冲性-PULSE、首次脉冲时间-TTFP、振幅-AMPL、最低点-NAD和曲线下面积-AUC）。此后，完成超声扫描以评估OR和AFN。使用门罗算法量化LH脉冲性；如果确定了时间、处理或交互作用的显著影响，则对不同时间的数据进行比较。处理组之间的LW、BCS（P>0.05）甚至LH（P>0.05）、PULSE（4.1 ± 0.9次脉冲/6小时）、NAD（0.47 ± 0.13 ng）和AUC（51.7 ± 18.6单位）均无差异。然而，补充β-胡萝卜素组的OR（1.57对0.87 ± 0.18单位）和TOA（3.44对1.87 ± 0.45单位）伴随着TTFP的降低（33对126 ± 31.9分钟）和AMPL的增加（0.55对0.24 ± 0.9 ng），差异有统计学意义（P<0.05），可能是通过GnRH-LH增强途径和（或）对卵巢水平的直接作用。这些结果对于加快山羊非季节性繁殖结果具有重要意义，同时可能具有转化应用价值。
