Du Nanshan, Yang Qian, Guo Hui, Xue Lu, Fu Ruike, Dong Xiaoxing, Dong Han, Guo Zhixin, Zhang Tao, Piao Fengzhi, Shen Shunshan
College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China.
College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.
Biology (Basel). 2022 Jul 8;11(7):1028. doi: 10.3390/biology11071028.
To gain insights into the roles of beneficial PGPR in controlling soil-borne disease, we adopted a metabolomics approach to investigate the beneficial impacts of NSY50 on cucumber seedling roots under the pathogen of f. sp. (FOC). We found that NSY50 pretreatment (NSY50 + FOC) obviously reduced the production of reactive oxygen species (ROS). Untargeted metabolomic analysis revealed that 106 metabolites responded to NSY50 and/or FOC inoculation. Under FOC stress, the contents of root osmotic adjustment substances, such as proline and betaine were significantly increased, and dehydroascorbic acid and oxidized glutathione (GSH) considerably accumulated. Furthermore, the contents of free amino acids such as tryptophan, phenylalanine, and glutamic acid were also significantly accumulated under FOC stress. Similarly, FOC stress adversely affected glycolysis and the tricarboxylic acid cycles and transferred to the pentose phosphate pathway. Conversely, NSY50 + FOC better promoted the accumulation of α-ketoglutaric acid, ribulose-5-phosphate, and 7-phosphosodiheptanone compared to FOC alone. Furthermore, NSY50 + FOC activated GSH metabolism and increased GSH synthesis and metabolism-related enzyme activity and their encoding gene expressions, which may have improved redox homoeostasis, energy flow, and defense ability. Our results provide a novel perspective to understanding the function of NSY50, accelerating the application of this beneficial PGPR in sustainable agricultural practices.
为深入了解有益植物根际促生细菌（PGPR）在控制土传病害中的作用，我们采用代谢组学方法研究了NSY50在尖孢镰刀菌古巴专化型（FOC）病原菌侵染下对黄瓜幼苗根系的有益影响。我们发现，NSY50预处理（NSY50 + FOC）明显降低了活性氧（ROS）的产生。非靶向代谢组学分析表明，有106种代谢物对NSY50和/或FOC接种有响应。在FOC胁迫下，根渗透调节物质如脯氨酸和甜菜碱的含量显著增加，脱氢抗坏血酸和氧化型谷胱甘肽（GSH）大量积累。此外，在FOC胁迫下，色氨酸、苯丙氨酸和谷氨酸等游离氨基酸的含量也显著积累。同样，FOC胁迫对糖酵解和三羧酸循环产生不利影响，并转向磷酸戊糖途径。相反，与单独的FOC相比，NSY50 + FOC能更好地促进α-酮戊二酸、5-磷酸核酮糖和7-磷酸景天庚酮糖的积累。此外，NSY50 + FOC激活了GSH代谢，增加了GSH合成及代谢相关酶的活性及其编码基因的表达，这可能改善了氧化还原稳态、能量流动和防御能力。我们的研究结果为理解NSY50的功能提供了新的视角，加速了这种有益PGPR在可持续农业实践中的应用。
