P. G. Department of Biosciences, Sardar Patel University, Gujarat, India.
Department of Plant Pathology and Ecology, The Connecticut Agricultural Experiment Station, New Haven, CT, USA.
J Appl Microbiol. 2021 Sep;131(3):1417-1430. doi: 10.1111/jam.15019. Epub 2021 Feb 25.
To characterize the mechanisms by which bacteria in the peanut rhizosphere promote plant growth and suppress Aspergillus niger, the fungus that causes collar rot of peanut.
In all, 131 isolates cultured from the peanut rhizosphere were assayed for growth promotion in a seedling germination assay. The most effective isolate, RR18, was identified as Burkholderia sp. by 16S sequencing analysis. RR18 reduced collar rot disease incidence and increased the germination rate and biomass of peanut seeds, and had broad-spectrum antifungal activity. Quantitative analyses showed that RR18 induced long-lasting accumulation of jasmonic acid, salicylic acid and phenols, and triggered the activity of six defence enzymes related to these changes. Comparative proteomic analysis of treated and untreated seedlings revealed a clear induction of four abundant proteins, including a member of the pre-chorismate pathway, a regulator of clathrin-coated vesicles, a transcription factor and a hypothetical protein.
Burkholderia sp. RR18 promotes peanut growth and disease resistance, and stably induces two distinct defence pathways associated with systemic resistance.
This study demonstrates that a strain of the Burkholderia cepacia complex can elicit both salicylic- and jasmonic-acid-mediated defences, in addition to having numerous other beneficial properties.
描述花生根际细菌促进植物生长和抑制导致花生茎腐病的黑曲霉的机制。
从花生根际共培养了 131 株菌，通过幼苗发芽试验测定其生长促进作用。最有效的分离株 RR18 通过 16S 测序分析鉴定为伯克霍尔德氏菌属。RR18 降低了茎腐病的发病率，提高了花生种子的发芽率和生物量，具有广谱的抗真菌活性。定量分析表明，RR18 诱导了持久的茉莉酸、水杨酸和酚类物质的积累，并触发了与这些变化相关的六种防御酶的活性。对处理和未处理的幼苗进行比较蛋白质组学分析，发现了四个丰富蛋白的明显诱导，包括预分支酸途径的一个成员、网格蛋白包被小泡的调节剂、一个转录因子和一个假设蛋白。
伯克霍尔德氏菌 RR18 促进了花生的生长和抗病性，并稳定地诱导了与系统抗性相关的两种不同的防御途径。
本研究表明，伯克霍尔德氏菌复合群的一株菌株可以引发水杨酸和茉莉酸介导的防御，此外还具有许多其他有益的特性。
