Kan Yuting, Zhong Ying, Jawad Muhammad, Chen Xiao, Liu Dong, Ren Mingchun, Xu Gangchun, Gui Lang, Li Mingyou
Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Biology (Basel). 2022 Jul 13;11(7):1049. doi: 10.3390/biology11071049.
Coilia nasus is an important economic anadromous migratory fish of the Yangtze River in China. In recent years, overfishing and the deterioration of the ecological environment almost led to the extinction of the wild resources of C.nasus. Thus, there is an urgent need to protect this endangered fish. Recently, cell lines derived from fish have proven a promising tool for studying important aspects of aquaculture. In this study, a stable C. nasus gonadal somatic cell line (CnCSC) was established and characterized. After over one year of cell culture (>80 passages), this cell line kept stable growth. RT-PCR results revealed that the CnGSC expressed some somatic cell markers such as clu, fshr, hsd3β, and sox9b instead of germ cell markers like dazl, piwi, and vasa. The strong phagocytic activity of CnGSC suggested that it contained a large number of Sertoli cells. Interestingly, CnGSC could induce medaka spermatogonial cells (SG3) to differentiate into elongated spermatids while co-cultured together. In conclusion, we established a C. nasus gonadal somatic cell line capable of sperm induction in vitro. This research provides scientific evidence for the long-term culture of a gonadal cell line from farmed fish, which would lay the foundation for exploring the regulatory mechanisms between germ cells and somatic cells in fish.
刀鲚是中国长江重要的经济溯河洄游性鱼类。近年来，过度捕捞和生态环境恶化几乎导致刀鲚野生资源灭绝。因此，迫切需要保护这种濒危鱼类。最近，鱼类来源的细胞系已被证明是研究水产养殖重要方面的一种有前景的工具。在本研究中，建立并鉴定了一种稳定的刀鲚性腺体细胞系（CnCSC）。经过一年多的细胞培养（>80代），该细胞系保持稳定生长。RT-PCR结果显示，CnGSC表达一些体细胞标志物，如clu、fshr、hsd3β和sox9b，而不表达生殖细胞标志物，如dazl、piwi和vasa。CnGSC的强吞噬活性表明它含有大量支持细胞。有趣的是，CnGSC与青鳉精原细胞（SG3）共培养时可诱导其分化为延长型精子细胞。总之，我们建立了一种能够在体外诱导精子形成的刀鲚性腺体细胞系。本研究为养殖鱼类性腺细胞系的长期培养提供了科学依据，为探索鱼类生殖细胞与体细胞之间的调控机制奠定了基础。
