环状 RNA 谱分析揭示 circ880 在日本青鳉性腺中作为 miR-375-3p 海绵的功能。
CircRNA profiling reveals circ880 functions as miR-375-3p sponge in medaka gonads.
机构信息
International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
出版信息
Comp Biochem Physiol Part D Genomics Proteomics. 2021 Jun;38:100797. doi: 10.1016/j.cbd.2021.100797. Epub 2021 Feb 27.
Circular RNAs (circRNAs) have been regarded as regulators in the biological processes of various species. However, there is no report about circRNAs in the gonads of model fish medaka (Oryzias latipes). In this study, 1157 and 1570 circRNAs were obtained in the ovary and testis by RNA-sequencing. The characteristics of circRNAs were explored in sequence length, exon composition, and chromosome position. 24 circRNAs were significantly up or down-regulated in the testis compared to the ovary, 9 of which were verified by qRT-PCR. Interestingly, circ452 was highly expressed in the testis while circ880 expression exhibited sexual dimorphism. In situ hybridization (ISH) revealed that circ452 and circ880 were expressed in meiotic germ cells, and circ880 was also abundant in spermatogonia. In addition, dual-luciferase reporter assay manifested that circ880 and Oldnd can combine with miR-375-3p. Overall, these results provide emerging circRNA libraries and open new avenues for future investigation of circRNAs in medaka.
环状 RNA（circRNAs）已被认为是各种物种生物过程的调节剂。然而，在模式鱼类斑马鱼（Oryzias latipes）的性腺中尚未有关于 circRNAs 的报道。在本研究中，通过 RNA 测序在卵巢和睾丸中分别获得了 1157 和 1570 个 circRNAs。在序列长度、外显子组成和染色体位置方面探索了 circRNAs 的特征。与卵巢相比，在睾丸中有 24 个 circRNAs 显著上调或下调，其中 9 个通过 qRT-PCR 进行了验证。有趣的是，circ452 在睾丸中高表达，而 circ880 的表达则表现出性别二态性。原位杂交（ISH）显示 circ452 和 circ880 在减数分裂生殖细胞中表达，circ880 在精原细胞中也很丰富。此外，双荧光素酶报告基因实验表明 circ880 和 Oldnd 可以与 miR-375-3p 结合。总体而言，这些结果提供了新兴的 circRNA 文库，并为未来对斑马鱼 circRNAs 的研究开辟了新途径。
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