Yao Meixue, Liu Mengda, Chen Xia, Li Jianjun, Li Yan, Wei Yu Run, Liu Yong, Yang Kang Long, Duan Xiaoxiao, Shao Weixing, Sun Xiangxiang, Fan Xiaoxu, Sun Shufang, Tian Lili, Yin Dehui, Sun Mingjun
Key Laboratory of Human Genetics and Environmental Medicine, School of Public Health, Xuzhou Medical University, Xuzhou, 221004, People's Republic of China.
Laboratory of Zoonoses, China Animal Health and Epidemiology Center, Qingdao, 266032, People's Republic of China.
Infect Drug Resist. 2022 Sep 7;15:5301-5308. doi: 10.2147/IDR.S374432. eCollection 2022.
Brucellosis is one of the most important zoonotic diseases in the world. Canine brucellosis, caused mainly by , is seriously neglected, and there is a lack of accurate diagnostic tools.
In this study, to compare BP26, Omp25, Omp31 and a multiepitope-based fusion protein in the serological detection of canine brucellosis, using 34 brucellosis-positive dog sera and 62 negative control sera, the outer membrane proteins Omp31, BP26, Omp25 and a multiepitope-based fusion protein were evaluated by iELISA for their potential use as antigens in the serological diagnosis of canine brucellosis.
The results showed that the multiepitope-based fusion protein performed best in distinguishing brucellosis-positive and brucellosis-negative dog sera, with a positive predictive value (PPV) of 100% and a negative predictive value (NPV) of 98.41%. BP26 and Omp31 showed excellent sensitivity in detecting brucellosis-positive dog sera, but their cross reaction to sera infected with and may hinder their application as diagnostic reagents. Omp25 lacked sufficient sensitivity and showed limited ability in distinguishing positive and negative dog sera.
The multiepitope-based fusion protein can be used as an ideal antigen for serologically diagnosing canine brucellosis currently prevalent worldwide.
布鲁氏菌病是世界上最重要的人畜共患病之一。主要由[未提及具体病原体]引起的犬布鲁氏菌病严重被忽视，且缺乏准确的诊断工具。
在本研究中，为比较BP26、Omp25、Omp31和一种基于多表位的融合蛋白在犬布鲁氏菌病血清学检测中的性能，使用34份布鲁氏菌病阳性犬血清和62份阴性对照血清，通过间接酶联免疫吸附测定（iELISA）评估外膜蛋白Omp31、BP26、Omp25和一种基于多表位的融合蛋白作为抗原在犬布鲁氏菌病血清学诊断中的潜在用途。
结果表明，基于多表位的融合蛋白在区分布鲁氏菌病阳性和阴性犬血清方面表现最佳，阳性预测值（PPV）为100%，阴性预测值（NPV）为98.41%。BP26和Omp31在检测布鲁氏菌病阳性犬血清时表现出优异的敏感性，但它们与感染[未提及具体病原体]和[未提及具体病原体]的血清发生交叉反应，这可能会阻碍它们作为诊断试剂的应用。Omp25缺乏足够的敏感性，在区分阳性和阴性犬血清方面能力有限。
基于多表位的融合蛋白可作为目前在全球流行的犬布鲁氏菌病血清学诊断的理想抗原。
