Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
College of Life Science and Food Engineering, Hebei University of Engineering, Handan 056038, China.
Biosensors (Basel). 2024 Aug 10;14(8):388. doi: 10.3390/bios14080388.
Brucellosis is a global problem, with the causative agent being the genus . can cause undulant fever in dogs, which is a zoonotic disease that can spread not only among dogs but also to humans. This poses a public health threat to society. In this study, a rapid and straightforward immune colloidal gold test strip was developed for the diagnosis of canine brucellosis through the detection of anti-LPS antibodies in serum samples. Rabbit anti-canine IgG conjugated with colloidal gold was employed as the colloidal gold-labeled antibody. The extracted high-purity R-LPS was employed as the capture antigen in the test line (T-line), while goat anti-rabbit IgG was utilized as the capture antibody in the control line (C-line). The colloidal gold strip exhibited high specificity in the detection of brucellosis, with no cross-reaction observed with the common clinical canine diseases caused by Canine coronavirus (CCV), Canine distemper virus (CDV), and Canine parvovirus (CPV). In comparison to the commercial iELISA kit, the sensitivity and specificity of the colloidal gold test strip were found to be 95.23% and 98.76%, respectively. The diagnostic coincidence rate was 98.47%. The findings of this study indicate that colloidal gold test strips may be employed as a straightforward, expeditious, sensitive, and specific diagnostic instrument for the identification of canine brucellosis, particularly in resource-limited regions.
布鲁氏菌病是一个全球性问题，其病原体为. 可引起犬只出现波浪热，这是一种人畜共患病，不仅可以在犬只之间传播，还可以传播给人类。这对社会的公共卫生构成了威胁。在这项研究中，通过检测血清样本中的抗 LPS 抗体，开发了一种快速简便的免疫胶体金测试条，用于诊断犬布鲁氏菌病。兔抗犬 IgG 与胶体金偶联作为胶体金标记抗体。提取的高纯度 R-LPS 作为测试线（T 线）的捕获抗原，而山羊抗兔 IgG 作为对照线（C 线）的捕获抗体。胶体金条在检测布鲁氏菌病方面表现出高度特异性，与犬冠状病毒（CCV）、犬瘟热病毒（CDV）和犬细小病毒（CPV）等常见临床犬病无交叉反应。与商业 iELISA 试剂盒相比，胶体金测试条的灵敏度和特异性分别为 95.23%和 98.76%。诊断符合率为 98.47%。本研究结果表明，胶体金测试条可作为一种简便、快捷、敏感、特异的犬布鲁氏菌病诊断工具，尤其适用于资源有限的地区。
