Ross David, Tonner Peter D, Vasilyeva Olga B
National Institute of Standards and Technology, Gaithersburg, MD, USA.
Synth Biol (Oxf). 2022 Aug 10;7(1):ysac013. doi: 10.1093/synbio/ysac013. eCollection 2022.
Microbial cell culture is one of the most commonly performed protocols for synthetic biology, and laboratories are increasingly using 96-well plates and laboratory automation systems for cell culture. Here, we describe a method for reproducible microbial culture using laboratory automation systems, including automated liquid handling, automated plate sealing and de-sealing, automated incubation and measurement of growing cultures. We discuss the key considerations that, in our experience, are important for reproducibility and present statistical analyses of data from 150 automated microbial growth experiments performed over 27 months using our automated method.
微生物细胞培养是合成生物学中最常用的实验方案之一，实验室越来越多地使用96孔板和实验室自动化系统进行细胞培养。在此，我们描述了一种使用实验室自动化系统进行可重复微生物培养的方法，包括自动液体处理、自动封板和开封板、自动培养以及对生长中的培养物进行测量。我们讨论了根据经验对可重复性很重要的关键注意事项，并展示了使用我们的自动化方法在27个月内进行的150次自动化微生物生长实验数据的统计分析。
