MiR-203a-3p、miR-204-3p、miR-222-3p作为甲状腺肿瘤谱系的有用诊断和预后工具。

MiR-203a-3p, miR-204-3p, miR-222-3p as useful diagnostic and prognostic tool for thyroid neoplasia spectrum.

作者信息

Stojanović Stefana, Dobrijević Zorana, Šelemetjev Sonja, Đorić Ilona, Janković Miljuš Jelena, Živaljević Vladan, Išić Denčić Tijana

机构信息

Department for Endocrinology and Radioimmunology, Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Banatska 31b, 11080, Belgrade, Serbia.

Department for Metabolism, Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Banatska 31b, 11080, Belgrade, Serbia.

出版信息

Endocrine. 2023 Jan;79(1):98-112. doi: 10.1007/s12020-022-03185-7. Epub 2022 Sep 14.

DOI:10.1007/s12020-022-03185-7

PMID:36103016

Abstract

PURPOSE

The challenge in the diagnosis and treatment of thyroid carcinoma is to correctly classify neoplasias with overlapping features and to identify the high-risk patients among those with a less aggressive form, in order to personalize the treatment of thyroid carcinoma patients accordingly.

METHODS

MiR-203a-3p, miR-204-3p, and miR-222-3p levels were determined in 99 cases of thyroid neoplasias (77 papillary thyroid carcinomas (PTC) of diverse variants, 12 follicular thyroid adenomas (FTA) and 10 nodular goiters (NG)) along with 99 adjacent non-malignant thyroid tissues using quantitative RT-PCR. The results were evaluated in comparison with the clinicopathological features of the patients and available TCGA data.

RESULTS

Down-regulated miR-203a-3p indicates the presence of thyroid tumor (PTC or FTA) with high sensitivity (75%) and specificity (73%), while its up-regulation indicates NG. If miR-203a-3p is down-regulated, up-regulated miR-204-3p with high sensitivity (83.3%) and specificity (74.4%) indicates FTA presence, while up-regulated miR-222-3p, with high sensitivity (76.6%) and specificity (75.0%), points to PTC. The expression of miR-204-3p and miR-222-3p depends on the PTC subtype (P < 0.05). While the deregulated expression of tested miRs is associated with a long-range of unfavorable clinicopathological parameters of PTC, only abundant expression of miR-222-3p may be used as an independent predictive factor for the presence of extrathyroid invasion and advanced pTNM stage of PTC (P < 0.05).

CONCLUSION

Successive evaluation of miR-203a-3p, miR-204-3p, and miR-222-3p expression can help in the differential diagnosis of thyroid neoplasias. A high relative value of miR-222-3p expression is an independent predictive factor for the presence of extrathyroid invasion and advanced pTNM stage of PTC. The panel consisting of miR-203a-3p, miR-204-3p, and miR-222-3p could be used as a diagnostic and prognostic tool for personalizing the treatment of thyroid cancer patients.

摘要

目的

甲状腺癌诊断和治疗中的挑战在于正确分类具有重叠特征的肿瘤，并在侵袭性较低的患者中识别出高危患者，以便据此对甲状腺癌患者进行个性化治疗。

方法

采用定量逆转录聚合酶链反应（qRT-PCR）测定99例甲状腺肿瘤（77例不同亚型的乳头状甲状腺癌（PTC）、12例滤泡性腺瘤（FTA）和10例结节性甲状腺肿（NG））以及99例相邻的非恶性甲状腺组织中miR-203a-3p、miR-204-3p和miR-222-3p的水平。将结果与患者的临床病理特征及可用的TCGA数据进行比较评估。

结果

miR-203a-3p表达下调提示存在甲状腺肿瘤（PTC或FTA），敏感性高（75%），特异性高（73%），而其表达上调提示为NG。若miR-203a-3p表达下调，miR-204-3p表达上调，敏感性高（83.3%），特异性高（74.4%），提示存在FTA，而miR-222-3p表达上调，敏感性高（76.6%），特异性高（75.0%），提示为PTC。miR-204-3p和miR-222-3p的表达取决于PTC亚型（P<0.05）。虽然所检测的miRs表达失调与PTC的一系列不良临床病理参数相关，但只有miR-222-3p的高表达可作为PTC甲状腺外侵犯和高级别pTNM分期的独立预测因素（P<0.05）。

结论

对miR-203a-3p、miR-204-3p和miR-222-3p表达进行连续评估有助于甲状腺肿瘤的鉴别诊断。miR-222-3p表达的高相对值是PTC甲状腺外侵犯和高级别pTNM分期的独立预测因素。由miR-203a-3p、miR-204-3p和miR-222-3p组成的检测组合可作为甲状腺癌患者个性化治疗的诊断和预后工具。