International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2022 Nov;39(11):1843-1854. doi: 10.1080/19440049.2022.2120635. Epub 2022 Sep 14.
In this study, we developed a monoclonal antibody (mAb)-based colloidal gold immunochromatography assay (CG-ICA) strip for the rapid and sensitive detection of flubendiamide (FBD) in food samples. Our anti-FBD mAb 2B1 was highly specific and had a half-maximal inhibitory concentration (IC) of 1.55 ng/mL under optimal conditions. It belongs to the IgG2a isotype and has a K value of 1.52 × 10 mol/L. The strip provided a visual detection limit of 50 ng/g for both grapes and tomatoes, with a cut-off value of 1,000 ng/g and these qualitative results were observed within 15 min. For quantitative analysis, the calculated detection limits were 6 ng/g and 5 ng/g in grapes and tomatoes, respectively. The average recoveries of FBD ranged from 88.8% to 111.8% in grapes and 94.5% to 110.6% in tomatoes. The proposed strip assay is highly practical for screening FDB in food samples.
在这项研究中，我们开发了一种基于单克隆抗体（mAb）的胶体金免疫层析检测条（CG-ICA），用于快速灵敏地检测食品样品中的氟苯虫酰胺（FBD）。我们的抗 FBD mAb 2B1 在最佳条件下具有高度特异性，半数最大抑制浓度（IC）为 1.55ng/mL。它属于 IgG2a 同种型，K 值为 1.52×10-9mol/L。该检测条在葡萄和番茄中的目视检测限均为 50ng/g，截断值为 1,000ng/g，在 15 分钟内观察到这些定性结果。对于定量分析，在葡萄和番茄中计算出的检测限分别为 6ng/g 和 5ng/g。FBD 在葡萄中的平均回收率范围为 88.8%至 111.8%，在番茄中的回收率为 94.5%至 110.6%。该条带检测方法在筛选食品样品中的 FDB 方面非常实用。
