Kasyanova Yu V, Vasyukova O V, Okorokov P L, Zuraeva Z T, Bezlepkina O B
Endocrinology Research Center.
Probl Endokrinol (Mosk). 2022 Jul 24;68(4):102-110. doi: 10.14341/probl13138.
Myokines are synthesized by myocytes and released into the bloodstream in response to muscle fiber contraction. They have a positive effect on carbohydrate and lipid metabolism, muscle mass growth, osteogenesis, increase tissue sensitivity to insulin, counteract inflammation of adipose tissue. The study of their secretion in response to physical activity (FA) can help to personalize the therapy of obesity.
to study the features of the secretion of myokines in children with constitutionally exogenous obesity during physical activity of different duration and intensity and to evaluate their relationship with the parameters of the body composition.
26 children (10 boys and 16 girls) were included in the study 15 [13; 16] years old, SDS BMI: +2.91 [2.24; 3.29], with sexual development according to Tanner 4-5. Two groups of 13 people were formed by random distribution. Group I performed FA (walking on a treadmill under the control of heart rate) of different duration: 30 and 60 minutes at the same intensity (less than 3 metabolic equivalents (MET)). Group II - FA of different intensity: low - less than 3 METH and moderate - 3-6 METH with the same duration of 45 minutes. Commercial kits for enzyme immunoassay were used to determine the level of myokines. The assessment of the compositional composition of the body was carried out by bioimpedance analysis (analyzer In Body 770, South Korea) in the morning, on an empty stomach. Statistical processing was carried out using STATISTICA v.12.0 (StatSoftInc., USA). The results are presented in the form of median (Me) and quartiles (Q1; Q3) corresponding to 25 and 75 percentiles. The critical significance level (p) was assumed to be <0.05.
moderate intensity FA leads to a maximum increase in the level of myokines: interleukin-6 (IL-6) by 215.7% and decorin by 34.3%, a decrease in the level of irisin by 16.5%. An hour-long low-intensity workout leads to a moderate increase in the level of IL-6 by 80.5%, to a decrease in the level of irisin by 31.1%. Myostatin increases equally both after 60-minute FA and after moderate intensity FA by 30.9% and 31.8%, respectively. Short low-intensity FA (lasting 30 minutes) it is not accompanied by a significant increase in the expression of myokines. The relationship between the amount of muscle (r=0.65), lean (r=0.62), fat-free mass (r=0.64) and the level of decorin after FA was noted. There was no statistically significant relationship between the parameters of the body composition and the levels of IL-6, myostatin, and irisin. There were no gender differences in both basal and stimulated myokine secretion.
Moderate intensity FA and low intensity 60-minute FA are most effective for obese children. A 30-minute low-intensity FN is insufficient to increase the secretion of myokines by skeletal muscles.
肌动蛋白由肌细胞合成，并在肌纤维收缩时释放到血液中。它们对碳水化合物和脂质代谢、肌肉质量增长、骨生成具有积极作用，可提高组织对胰岛素的敏感性，对抗脂肪组织炎症。研究其在体育活动（PA）中的分泌情况有助于实现肥胖症治疗的个性化。
研究不同持续时间和强度的体育活动期间，体质性外源性肥胖儿童的肌动蛋白分泌特征，并评估其与身体成分参数的关系。
26名儿童（10名男孩和16名女孩）参与研究，年龄15[13；16]岁，SDS BMI：+2.91[2.24；3.29]，性发育处于坦纳4-5期。通过随机分组形成两组，每组13人。第一组进行不同持续时间的体育活动（在心率控制下在跑步机上行走）：30分钟和60分钟，强度相同（低于3代谢当量（MET））。第二组进行不同强度的体育活动：低强度 - 低于3 MET，中等强度 - 3-6 MET，持续时间均为45分钟。使用酶免疫分析商业试剂盒测定肌动蛋白水平。早晨空腹时通过生物电阻抗分析（韩国In Body 770分析仪）对身体成分进行评估。使用STATISTICA v.12.0（美国StatSoftInc.）进行统计处理。结果以中位数（Me）和对应第25和75百分位数的四分位数（Q1；Q3）形式呈现。临界显著性水平（p）设定为<0.05。
中等强度的体育活动导致肌动蛋白水平最大幅度升高：白细胞介素-6（IL-6）升高215.7%，核心蛋白聚糖升高34.3%，鸢尾素水平降低16.5%。长达一小时的低强度锻炼导致IL-6水平适度升高80.5%，鸢尾素水平降低31.1%。60分钟的体育活动和中等强度的体育活动后，肌肉生长抑制素分别升高30.9%和31.8%。短时间低强度的体育活动（持续30分钟）并未伴随肌动蛋白表达的显著增加。观察到体育活动后肌肉量（r = 0.65）、瘦体重（r = 0.62）、去脂体重（r = 0.64）与核心蛋白聚糖水平之间的关系。身体成分参数与IL-6、肌肉生长抑制素和鸢尾素水平之间无统计学显著关系。基础和刺激后的肌动蛋白分泌均无性别差异。
中等强度的体育活动和60分钟的低强度体育活动对肥胖儿童最有效。30分钟的低强度体育活动不足以增加骨骼肌肌动蛋白的分泌。
