Dong Lina, Liang Mujiao, Guo Zhongwei, Wang Anyang, Cai Gangpei, Yuan Tianying, Mi Shengli, Sun Wei
Macromolecular Platforms for Translational Medicine and Bio-Manufacturing Laboratory, Tsinghua-Berkeley Shenzhen Institute, Shenzhen 518055, P.R. China.
Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.
Int J Bioprint. 2022 Jun 8;8(3):578. doi: 10.18063/ijb.v8i3.578. eCollection 2022.
In nature, many biological tissues are composed of oriented structures, which endow tissues with special properties and functions. Although traditional hydrogels can achieve a high level of biomimetic composition, the orderly arrangement of internal structures remains a challenge. Therefore, it is of great significance to synthesize hydrogels with oriented structures easily and quickly. In this study, we first proposed and demonstrated a fabrication process for producing a well-ordered and dual-responsive cellulose nanofibers + hyaluronic acid methacrylate (CN+HAMA) hydrogels through an extrusion-based three-dimensional (3D) printing process. CN in the CN+HAMA hydrogels are directionally aligned after extrusion due to shear stress. In addition, the synthesized hydrogels exhibited responsive behaviors to both temperature and ultraviolet light. Since the temperature-responsiveness is reversible, the hydrogels can transit between the gelation and solution states while retaining their original qualities. Furthermore, the developed well-oriented CN+HAMA hydrogels induced directional cell growth, paving the way for potential applications in ordered biological soft-tissue repair.
在自然界中，许多生物组织由定向结构组成，这些结构赋予组织特殊的性质和功能。尽管传统水凝胶能够实现高度的仿生组成，但内部结构的有序排列仍然是一个挑战。因此，轻松快速地合成具有定向结构的水凝胶具有重要意义。在本研究中，我们首次提出并展示了一种通过基于挤出的三维（3D）打印工艺制备有序且双响应的纤维素纳米纤维+甲基丙烯酸透明质酸（CN+HAMA）水凝胶的制造工艺。由于剪切应力，CN+HAMA水凝胶中的CN在挤出后定向排列。此外，合成的水凝胶对温度和紫外线均表现出响应行为。由于温度响应是可逆的，水凝胶可以在凝胶态和溶液态之间转变，同时保持其原始性质。此外，所开发的高度定向的CN+HAMA水凝胶诱导细胞定向生长，为有序生物软组织修复的潜在应用铺平了道路。
