Nadalian Banafsheh, Nadalian Bahareh, Houri Hamidreza, Shahrokh Shabnam, Abdehagh Mohammad, Yadegar Abbas, Ebrahimipour Gholamhossein
Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Front Med (Lausanne). 2022 Aug 29;9:985300. doi: 10.3389/fmed.2022.985300. eCollection 2022.
Although the etiopathogenesis of inflammatory bowel disease (IBD) is still poorly understood, has been described as a potential causative microorganism in IBD pathogenesis and also disease progression, offering a potential therapeutic target for disease management. Therefore, we conducted this study to investigate the pathotypes, phylogenetic groups, and antimicrobial resistance of isolates from patients with IBD in Iran.
Fecal and biopsy colonic samples were collected from IBD patients experiencing flare-up episodes referred to Taleghani hospital in Tehran, Iran, between August 2020 and January 2021. Identification of strains was performed based on biochemical and molecular methods. Antibiotic susceptibility testing was performed as recommended by the Clinical and Laboratory Standards Institute. Phylogrouping and pathotyping of each isolate were carried out using polymerase chain reaction (PCR) and multilocus sequence typing (MLST) assays.
A total of 132 non-duplicate strains were isolated from 113 IBD patients, including 96 ulcerative colitis (UC), and 17 Crohn's disease (CD) patients. In our study, 55% of CD-related and 70.5% of UC-related isolates were non-susceptible to at least three or more unique antimicrobial classes, and were considered as multidrug-resistant (MDR) strains. strains exhibited a high level of resistance to cefazolin, ampicillin, tetracycline, ceftazidime, ciprofloxacin, and cefotaxime. Enterotoxigenic (ETEC) and diffusely adherent (DAEC) were the most prevalent pathotypes, and groups B2 and D were the predominant phylogroups.
In the present study, we found that strains that colonize the gut of Iranian patients with IBD most frequently belonged to phylogenetic groups B2 and D. We also conclude that isolates from IBD patients have been revealed to be resistant to commonly used antibiotics, in which most of them harbored strains that would be categorized as MDR.
尽管炎症性肠病（IBD）的发病机制仍未完全明确，但[某种微生物]已被描述为IBD发病机制及疾病进展中的潜在致病微生物，为疾病管理提供了一个潜在的治疗靶点。因此，我们开展了本研究，以调查伊朗IBD患者分离出的[该微生物]的致病型、系统发育群及抗菌药物耐药性。
2020年8月至2021年1月期间，从转诊至伊朗德黑兰塔莱加尼医院的处于疾病发作期的IBD患者中收集粪便和结肠活检样本。基于生化和分子方法对[该微生物]菌株进行鉴定。按照临床和实验室标准协会的建议进行抗生素敏感性测试。使用聚合酶链反应（PCR）和多位点序列分型（MLST）分析对每个分离株进行系统发育分组和致病型分型。
共从113例IBD患者中分离出132株非重复的[该微生物]菌株，其中包括96例溃疡性结肠炎（UC）患者和17例克罗恩病（CD）患者。在我们的研究中，55%的与CD相关的[该微生物]以及70.5%的与UC相关的分离株对至少三种或更多独特的抗菌药物类别不敏感，被视为多重耐药（MDR）菌株。[该微生物]菌株对头孢唑林、氨苄西林、四环素、头孢他啶、环丙沙星和头孢噻肟表现出高度耐药性。产肠毒素[该微生物]（ETEC）和弥漫性黏附[该微生物]（DAEC）是最常见的致病型，B2和D组是主要的系统发育群。
在本研究中，我们发现定植于伊朗IBD患者肠道的[该微生物]菌株最常属于系统发育群B2和D。我们还得出结论，IBD患者分离出的[该微生物]已显示出对常用抗生素耐药，其中大多数携带的菌株可归类为MDR。
