Nieuwenhuysen P, Clauwaert J
Biochemistry. 1978 Oct 3;17(20):4260-5. doi: 10.1021/bi00613a023.
Eukaryotic ribosomes were isolated from the cryptobiotic embryos and from the further-developed free-swimming nauplii of the brine shrimp Artemia salina. Analytical boundary sedimentation and photon correlation spectroscopy yielded, respectively, the standard sedimentation and diffusion coefficients at infinite dilution, s degrees 20,w = 81 +/- 1 S and D degrees 20,w = (1.41 +/- 0.02) x 10(-7) cm2/s, for the unfixed and formaldehyde-fixed ribosomes from different developmental stages and for ribosomes attached to a messenger RNA fragment. Also, the density increment was determined, from which the partial specific volume was derived (0.63 +/- 0.01 cm3/g). Combination of the different measured parameters gives accurate values for the molecular weight (3.8 +/- 0.1) x 106 and for size and solvation parameters. These results are compared with their counterparts for the smaller ribosomes from the prokaryote Escherichia coli.
真核生物核糖体是从处于隐生状态的卤虫胚胎以及进一步发育的自由游动无节幼体中分离得到的。通过分析型边界沉降法和光子相关光谱法，分别得到了不同发育阶段的未固定和甲醛固定核糖体以及附着在信使RNA片段上的核糖体在无限稀释时的标准沉降系数和扩散系数，即s°20,w = 81 ± 1 S和D°20,w = (1.41 ± 0.02)×10(-7) cm2/s。此外，还测定了密度增量，并由此得出了偏比容(0.63 ± 0.01 cm3/g)。综合不同的测量参数，得到了分子量为(3.8 ± 0.1)×106以及大小和溶剂化参数的准确值。将这些结果与原核生物大肠杆菌中较小核糖体的相应结果进行了比较。
