热带假丝酵母儿茶酚O-甲基转移酶的动力学及抑制研究
Kinetics and inhibition studies of catechol O-methyltransferase from the yeast Candida tropicalis.
作者信息
Veser J
出版信息
J Bacteriol. 1987 Aug;169(8):3696-700. doi: 10.1128/jb.169.8.3696-3700.1987.
Abstract
The Kms for esculetin and S-adenosyl-L-methionine for catechol O-methyltransferase from the yeast Candida tropicalis were 6.2 and 40 microM, respectively. S-Adenosyl-L-homocysteine was a very potent competitive inhibitor with respect to S-adenosyl-L-methionine, with a Ki of 6.9 microM. Of the catechol-related inhibitors, purpurogallin, with a Ki of 0.07 microM, showed the greatest inhibitory effect. Sulfhydryl group-blocking reagents, such as thiol-oxidizing 2-iodosobenzoic acid and mercaptide-forming p-chloromercuribenzoic acid, provided evidence for sulfhydryl groups in the active site of the enzyme. Yeast catechol O-methyltransferase is a metal-dependent enzyme and requires Mg2+ for full activity. Zn2+ and Mn2+ but not Ca2+ were able to substitute for Mg2+. Mn2+ showed optimal enzyme activation at concentrations 50- to 100-fold lower than those of Mg2+.
摘要
热带假丝酵母儿茶酚-O-甲基转移酶对七叶亭和S-腺苷-L-甲硫氨酸的米氏常数分别为6.2和40微摩尔。S-腺苷-L-高半胱氨酸是一种对S-腺苷-L-甲硫氨酸非常有效的竞争性抑制剂，其抑制常数为6.9微摩尔。在儿茶酚相关抑制剂中，抑制常数为0.07微摩尔的紫铆因显示出最大的抑制作用。巯基阻断剂，如氧化巯基的2-碘代苯甲酸和形成硫醇盐的对氯汞苯甲酸，为该酶活性位点中的巯基提供了证据。酵母儿茶酚-O-甲基转移酶是一种金属依赖性酶，需要Mg2+才能发挥全部活性。Zn2+和Mn2+能够替代Mg2+，但Ca2+不能。Mn2+在浓度比Mg2+低50至100倍时显示出最佳的酶激活作用。
相似文献
1
Kinetics and inhibition studies of catechol O-methyltransferase from the yeast Candida tropicalis.热带假丝酵母儿茶酚O-甲基转移酶的动力学及抑制研究
J Bacteriol. 1987 Aug;169(8):3696-700. doi: 10.1128/jb.169.8.3696-3700.1987.
2
Kinetic studies on the O-methylation of dopamine by human brain membrane-bound catechol O-methyltransferase.人脑膜结合儿茶酚-O-甲基转移酶对多巴胺O-甲基化的动力学研究。
Biochemistry. 1982 Apr 13;21(8):1740-2. doi: 10.1021/bi00537a006.
3
High-performance liquid chromatographic determination, with ultraviolet detection, of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine in rat tissues and simultaneously of normetanephrine and metanephrine for phenylethanolamine-N-methyltransferase or catechol-O-methyltransferase activities.高效液相色谱法结合紫外检测测定大鼠组织中的S-腺苷-L-甲硫氨酸和S-腺苷-L-高半胱氨酸，同时测定去甲变肾上腺素和变肾上腺素以评估苯乙醇胺-N-甲基转移酶或儿茶酚-O-甲基转移酶活性。
J Chromatogr. 1991 Jun 14;567(1):254-60. doi: 10.1016/0378-4347(91)80329-b.
4
O-Methylation of tea polyphenols catalyzed by human placental cytosolic catechol-O-methyltransferase.人胎盘胞质儿茶酚-O-甲基转移酶催化的茶多酚O-甲基化反应
Drug Metab Dispos. 2000 Sep;28(9):1024-30.
5
Kinetic and inhibition studies on catechol-O-methyltransferase affinity labelling by N-(3,4-dihydroxyphenyl)maleimide.N-(3,4-二羟基苯基)马来酰亚胺对儿茶酚-O-甲基转移酶亲和标记的动力学及抑制研究
Biochem J. 1992 Sep 15;286 ( Pt 3)(Pt 3):951-8. doi: 10.1042/bj2860951.
6
Immunocytochemical demonstration of catechol methyltransferase in Candida tropicalis.热带假丝酵母中儿茶酚甲基转移酶的免疫细胞化学证明
J Gen Microbiol. 1981 Sep;126(1):97-101. doi: 10.1099/00221287-126-1-97.
7
Purification and properties of a catechol methyltransferase of the yeast Candida tropicalis.热带假丝酵母儿茶酚甲基转移酶的纯化及性质
Z Naturforsch C Biosci. 1979 Sep-Oct;34(9-10):709-14. doi: 10.1515/znc-1979-9-1010.
8
Purification and properties of S-adenosyl-L-methionine: caffeic acid O-methyltransferase from leaves of spinach beet (Beta vulgaris L).来自菠菜甜菜（Beta vulgaris L.）叶片的S-腺苷-L-甲硫氨酸：咖啡酸O-甲基转移酶的纯化及性质
Biochim Biophys Acta. 1975 Oct 22;403(2):301-14. doi: 10.1016/0005-2744(75)90060-1.
9
[O-Methylation of epinephrine, 3,4-dihydroxybenzoic acid and 6,7-dihydroxycoumarin in yeasts (author's transl)].酵母中肾上腺素、3,4-二羟基苯甲酸和6,7-二羟基香豆素的O-甲基化作用（作者译）
Z Naturforsch C Biosci. 1976 Sep-Oct;31(9-10):509-13.
10
Photoaffinity labeling of catechol O-methyltransferase with 8-azido-S-adenosylmethionine.用8-叠氮基-S-腺苷甲硫氨酸对儿茶酚-O-甲基转移酶进行光亲和标记。
J Biol Chem. 1983 Feb 10;258(3):1747-51.
引用本文的文献
1
Characterization of a unique catechol-O-methyltransferase as a molecular drug target in parasitic filarial nematodes.鉴定独特儿茶酚-O-甲基转移酶作为寄生性丝虫的分子药物靶点。
PLoS Negl Trop Dis. 2024 Aug 30;18(8):e0012473. doi: 10.1371/journal.pntd.0012473. eCollection 2024 Aug.
2
Biotechnological applications of S-adenosyl-methionine-dependent methyltransferases for natural products biosynthesis and diversification.S-腺苷甲硫氨酸依赖性甲基转移酶在天然产物生物合成和多样化中的生物技术应用。
Bioresour Bioprocess. 2021 Aug 11;8(1):72. doi: 10.1186/s40643-021-00425-y.
3
Coumarin-Based Profluorescent and Fluorescent Substrates for Determining Xenobiotic-Metabolizing Enzyme Activities In Vitro.基于香豆素的前荧光和荧光底物用于测定体外的异生物质代谢酶活性。
Int J Mol Sci. 2020 Jul 1;21(13):4708. doi: 10.3390/ijms21134708.
4
Inhibitory Effect of Purpurogallin on Osteoclast Differentiation in Vitro through the Downregulation of c-Fos and NFATc1.没食子酸丙酯通过下调 c-Fos 和 NFATc1 抑制破骨细胞分化。
Int J Mol Sci. 2018 Feb 17;19(2):601. doi: 10.3390/ijms19020601.
5
Antiplatelet and antithrombotic activities of purpurogallin in vitro and in vivo.没食子酸丙酯在体內和体外用的抗血小板和抗血栓作用。
BMB Rep. 2014 Jul;47(7):376-81. doi: 10.5483/bmbrep.2014.47.7.195.
6
How metal substitution affects the enzymatic activity of catechol-o-methyltransferase.金属取代如何影响儿茶酚-O-甲基转移酶的酶活性。
PLoS One. 2012;7(10):e47172. doi: 10.1371/journal.pone.0047172. Epub 2012 Oct 8.
7
Bacterial Hen1 is a 3' terminal RNA ribose 2'-O-methyltransferase component of a bacterial RNA repair cassette.细菌 Hen1 是细菌 RNA 修复盒的 3' 末端 RNA 核糖 2'-O-甲基转移酶的组成部分。
RNA. 2010 Feb;16(2):316-23. doi: 10.1261/rna.1926510. Epub 2009 Dec 9.
8
Catechol-O-methyltransferase and its inhibitors in Parkinson's disease.儿茶酚-O-甲基转移酶及其抑制剂与帕金森病
CNS Drug Rev. 2007 Fall;13(3):352-79. doi: 10.1111/j.1527-3458.2007.00020.x.
9
Methylation of halogenated phenols and thiophenols by cell extracts of gram-positive and gram-negative bacteria.革兰氏阳性菌和革兰氏阴性菌细胞提取物对卤代酚和硫代酚的甲基化作用。
Appl Environ Microbiol. 1988 Feb;54(2):524-30. doi: 10.1128/aem.54.2.524-530.1988.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Enzymatic O-methylation of epinephrine and other catechols.肾上腺素及其他儿茶酚的酶促O-甲基化作用。
J Biol Chem. 1958 Sep;233(3):702-5.
3
Coumarins and phenolic acids in the uredospores of wheat stem rust.小麦秆锈病夏孢子中的香豆素和酚酸
Can J Microbiol. 1957 Oct;3(6):847-62. doi: 10.1139/m57-094.
4
The determination of enzyme inhibitor constants.酶抑制剂常数的测定
Biochem J. 1953 Aug;55(1):170-1. doi: 10.1042/bj0550170.
5
Kinetic studies on the O-methylation of dopamine by human brain membrane-bound catechol O-methyltransferase.人脑膜结合儿茶酚-O-甲基转移酶对多巴胺O-甲基化的动力学研究。
Biochemistry. 1982 Apr 13;21(8):1740-2. doi: 10.1021/bi00537a006.
6
Immunocytochemical demonstration of catechol methyltransferase in Candida tropicalis.热带假丝酵母中儿茶酚甲基转移酶的免疫细胞化学证明
J Gen Microbiol. 1981 Sep;126(1):97-101. doi: 10.1099/00221287-126-1-97.
7
Localization of catechol-O-methyltransferase in the leptomeninges, choroid plexus and ciliary epithelium: implications for the separation of central and peripheral catechols.儿茶酚-O-甲基转移酶在软脑膜、脉络丛和睫状上皮中的定位：对中枢和外周儿茶酚分离的意义。
Brain Res. 1981 Jan 12;204(2):353-60. doi: 10.1016/0006-8993(81)90594-1.
8
Distinct cellular localization of membrane-bound and soluble forms of catechol-O-methyltransferase in brain.脑中儿茶酚-O-甲基转移酶膜结合形式与可溶性形式的不同细胞定位。
J Neurochem. 1983 Jan;40(1):215-9. doi: 10.1111/j.1471-4159.1983.tb12673.x.
9
Immunological studies of catechol methyltransferase from the yeast Candida tropicalis.热带假丝酵母儿茶酚甲基转移酶的免疫学研究
Z Naturforsch C Biosci. 1980 Sep-Oct;35(9-10):712-6. doi: 10.1515/znc-1980-9-1009.
10
Magnesium and the regulation of germ-tube formation in Candida albicans.镁与白色念珠菌芽管形成的调节
J Gen Microbiol. 1984 Aug;130(8):1941-5. doi: 10.1099/00221287-130-8-1941.
Zotero 插件