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高效液相色谱法结合紫外检测测定大鼠组织中的S-腺苷-L-甲硫氨酸和S-腺苷-L-高半胱氨酸，同时测定去甲变肾上腺素和变肾上腺素以评估苯乙醇胺-N-甲基转移酶或儿茶酚-O-甲基转移酶活性。

High-performance liquid chromatographic determination, with ultraviolet detection, of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine in rat tissues and simultaneously of normetanephrine and metanephrine for phenylethanolamine-N-methyltransferase or catechol-O-methyltransferase activities.

作者信息

Guattari B

机构信息

Laboratoire d'Eutonologie, Hôpital Boucicaut, Paris, France.

出版信息

J Chromatogr. 1991 Jun 14;567(1):254-60. doi: 10.1016/0378-4347(91)80329-b.

DOI:10.1016/0378-4347(91)80329-b
PMID:1918253
Abstract

A high-performance liquid chromatographic method, with ultraviolet detection, for the determination of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine, and simultaneously of normetanephrine and metanephrine, is presented. The separation was carried out by reversed-phase ion-pair isocratic chromatography. This procedure was applied to the study of the content of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine in rat brain and adrenal glands and to the measurement of phenylethanolamine-N-methyltransferase or catechol-O-methyltransferase activities in rat adrenal gland.

摘要

本文介绍了一种采用紫外检测的高效液相色谱法，用于测定S-腺苷-L-甲硫氨酸和S-腺苷-L-高半胱氨酸，同时测定去甲变肾上腺素和变肾上腺素。分离采用反相离子对等度色谱法。该方法应用于大鼠脑和肾上腺中S-腺苷-L-甲硫氨酸和S-腺苷-L-高半胱氨酸含量的研究，以及大鼠肾上腺中苯乙醇胺-N-甲基转移酶或儿茶酚-O-甲基转移酶活性的测定。

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1
High-performance liquid chromatographic determination, with ultraviolet detection, of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine in rat tissues and simultaneously of normetanephrine and metanephrine for phenylethanolamine-N-methyltransferase or catechol-O-methyltransferase activities.高效液相色谱法结合紫外检测测定大鼠组织中的S-腺苷-L-甲硫氨酸和S-腺苷-L-高半胱氨酸，同时测定去甲变肾上腺素和变肾上腺素以评估苯乙醇胺-N-甲基转移酶或儿茶酚-O-甲基转移酶活性。
J Chromatogr. 1991 Jun 14;567(1):254-60. doi: 10.1016/0378-4347(91)80329-b.
2
Specific photoactivated covalent binding of S-adenosylmethionine to phenylethanolamine-N-methyl transferase.S-腺苷甲硫氨酸与苯乙醇胺-N-甲基转移酶的特异性光活化共价结合。
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Enzymatic radioimmunoassay of epinephrine, norepinephrine, metanephrine, and normetanephrine.
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A radiometric assay for phenylethanolamine N-methyltransferase and catechol O-methyltransferase in a single tissue sample: application to rat hypothalamic nuclei, pineal gland, and heart.
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Simultaneous analysis of catechol-O-methyl transferase activity, S-adenosylhomocysteine and adenosine.儿茶酚-O-甲基转移酶活性、S-腺苷同型半胱氨酸和腺苷的同步分析
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Assay of phenylethanolamine N-methyltransferase activity using high-performance liquid chromatography with ultraviolet absorbance detection.使用带紫外吸收检测的高效液相色谱法测定苯乙醇胺N-甲基转移酶活性。
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Phospholipid methylase activity, [3H]S-adenosyl-L-homocysteine binding, and S-adenosyl-L-methionine and S-adenosyl-L-homocysteine levels in rat brain during maturation.大鼠脑成熟过程中磷脂甲基酶活性、[3H]S-腺苷-L-高半胱氨酸结合以及S-腺苷-L-甲硫氨酸和S-腺苷-L-高半胱氨酸水平
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