Lü Dongbiao, Dong Yanlu, Yan Zizheng, Liu Xueying, Zhang Yongjun, Yang Daibin, He Kanglai, Wang Zhenying, Wang Ping, Yuan Xiangqun, Li Yiping
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Pest Manag Sci. 2023 Jan;79(1):173-182. doi: 10.1002/ps.7186. Epub 2022 Oct 5.
Spodoptera frugiperda is an important invasive agricultural pest that causes huge economic losses worldwide. Gut microorganisms play a vital role in host feeding, digestion, nutrition, immunity, growth and insecticide resistance. Illumina high-throughput sequencing was used to study the gut microbial community dynamics across the life cycle (egg, 1st to 6th instar larvae, pupae, and male and female adults) of S. frugiperda fed on maize leaves. Furthermore, the gut microbial community and food intake of the 5th instar S. frugiperda larvae were studied after feeding them antibiotics.
Enterobacteriaceae and Enterococcaceae dominated the gut during growth and feeding of the larvae. The relative abundance of Enterobacteriaceae was higher in the 4th and 6th instar larvae. With the increase in larval feeding, the relative abundance of Enterococcaceae gradually increased. In addition, principal coordinate analysis and linear discriminant effect size analysis confirmed differences in the structure of gut microbiota at different developmental stages. After antibiotic treatment, the relative abundance of Firmicutes, Proteobacteria and Fusobacteriota decreased. The relative abundance of Enterococcus and Klebsiella decreased significantly. Antibiotic treatment inhibited the gut flora of S. frugiperda, which decreased larval food intake and body weight gain, and prolonged the larval stage.
The composition of the gut bacterial community plays an important role in the growth, development, and feeding of S. frugiperda. The results have a certain theoretical value for the development of bio-pesticides targeting intestinal flora. © 2022 Society of Chemical Industry.
草地贪夜蛾是一种重要的入侵性农业害虫，在全球范围内造成巨大经济损失。肠道微生物在宿主取食、消化、营养、免疫、生长和抗药性方面发挥着至关重要的作用。本研究利用Illumina高通量测序技术，对取食玉米叶片的草地贪夜蛾整个生命周期（卵、1至6龄幼虫、蛹以及雌雄成虫）的肠道微生物群落动态进行了研究。此外，还研究了用抗生素饲喂5龄草地贪夜蛾幼虫后其肠道微生物群落和食物摄入量的变化。
肠杆菌科和肠球菌科在幼虫生长和取食期间在肠道中占主导地位。肠杆菌科在4龄和6龄幼虫中的相对丰度较高。随着幼虫取食量的增加，肠球菌科的相对丰度逐渐增加。此外，主坐标分析和线性判别效应大小分析证实了不同发育阶段肠道微生物群结构存在差异。抗生素处理后，厚壁菌门、变形菌门和梭杆菌门的相对丰度降低。肠球菌和克雷伯菌的相对丰度显著下降。抗生素处理抑制了草地贪夜蛾的肠道菌群，导致幼虫食物摄入量和体重增加减少，并延长了幼虫期。
肠道细菌群落的组成在草地贪夜蛾的生长、发育和取食中起着重要作用。该结果对于开发针对肠道菌群的生物农药具有一定的理论价值。© 2022化学工业协会。
