Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Balikesir University, Balikesir, Turkey.
Department Microbiology, Faculty of Veterinary Medicine, Balikesir University, Balikesir, Turkey.
Food Sci Technol Int. 2024 Jan;30(1):30-36. doi: 10.1177/10820132221125104. Epub 2022 Sep 13.
Shiga toxin-producing (STEC) has emerged as important enteric foodborne zoonotic pathogens of considerable public health significance worldwide. The aim of this study was to determine the prevalence of the top seven STEC serotypes and to identify these serotypes in samples of animal source foods and vegetables. A total of 294 samples including 84 meat samples, 135 milk and dairy product samples and 75 green leafy vegetable samples were tested. The samples were harvested in mTSB-broth pre-enriched with novobiocin and then were tested by amplifying 16S shiga toxin (), and genes using multiplex polymerase chain reaction (m-PCR) assay. A total of 260 (88.4%) samples were positive for and 29 (11.1%) of them were positive for shiga toxin, and genes. The positive samples were cultivated on CHROMAgar STEC and the colonies were evaluated for top seven STEC by m-PCR. The top seven STEC serotypes were detected in 27 (93.1%) of the samples: the STEC O111 serotype in 11 (40.7%) beef samples, STEC O45 in 3 (11.1%) chicken, STEC O145 in 6 (22.2%) parsley, 3 (11.1%) lettuce, 1 (3.7%) spinach, and 1 (3.7%) cheese, and STEC O103 in 2 (7.4%) lettuce samples. None of the samples was found positive for STEC O26, O121, and O157 serotypes. This study highlights the fact that the top seven STEC group poses a great risk in terms of food safety and public health in both animal source foods and vegetables.
产志贺毒素（STEC）已成为具有重要意义的食源性人畜共患病病原体，对全球公共卫生具有相当大的影响。本研究旨在确定七种主要 STEC 血清型的流行率，并确定动物源性食品和蔬菜样本中的这些血清型。共检测了 294 个样本，包括 84 个肉类样本、135 个奶及奶制品样本和 75 个绿叶蔬菜样本。将这些样本在含有新生霉素的 mTSB 肉汤中预富集，然后通过多重聚合酶链反应（m-PCR）检测 16S 志贺毒素（stx1 和 stx2）和 基因来进行测试。共有 260 个（88.4%）样本对 基因呈阳性，其中 29 个（11.1%）对志贺毒素和 stx2 基因呈阳性。将阳性样本接种于 CHROMAgar STEC 平板，通过 m-PCR 对这七种主要 STEC 进行评估。在 27 个样本中检测到了这七种主要 STEC 血清型：11 个（40.7%）牛肉样本为 STEC O111 血清型，3 个（11.1%）鸡肉样本为 STEC O45 血清型，6 个（22.2%）欧芹样本为 STEC O145 血清型，3 个（11.1%）生菜样本为 STEC O145 血清型，1 个（3.7%）菠菜样本为 STEC O145 血清型，1 个（3.7%）奶酪样本为 STEC O145 血清型，2 个（7.4%）生菜样本为 STEC O103 血清型。没有样本对 STEC O26、O121 和 O157 血清型呈阳性。本研究强调了一个事实，即这七种主要 STEC 血清型在动物源性食品和蔬菜中对食品安全和公共健康构成了巨大威胁。
