Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad 38000, Pakistan.
Department of Vegetable and Herbal Crops, University of Life Sciences in Lublin, 50A Doświadczalna Street, 20-280 Lublin, Poland.
Biomed Pharmacother. 2022 Nov;155:113702. doi: 10.1016/j.biopha.2022.113702. Epub 2022 Sep 15.
Herbal drugs offer an alternative approach for the treatment of diseases like asthma due to low cost and comparatively less adverse effects in contrast to synthetic drugs. Leaves of Quercus leucotrichophora are traditionally used for the treatment of asthma. The study was aimed to assess the anti-asthmatic activity of Quercus leucotrichophora (QL) methanolic (QLME) and aqueous extracts (QLAE) in ovalbumin-(OVA) induced asthma and chemical characterization of QL extract by High Performance Liquid Chromatography-Diode array detector (HPLC-DAD). Animals were inoculated with OVA (i.p) on day 1 and 14 followed by intranasal challenge on 27th and 29th day. Both extracts of QL at 600, 300 and 150 mg/kg and dexamethasone (2 mg/kg) l were administered consecutively from days 15-26 via oral gavage. The QL extracts notably reduced (p < 0.0001-p < 0.05) total and differential leukocyte counts in blood and BALF and serum IgE levels in contrast to disease control. Both extracts and Dex substantially improved activities of superoxide dismutase, catalase, and GSH, while reduced malondialdehyde level in treated mice. Treatment with extracts and Dex caused significant (p < 0.0001-p < 0.05) downregulation of tumor necrosis factor-α, interleukin-4, - 5, - 13, - 6, - 1β, and NF-κB whereas, increased expression of Aquaporin (AQP) 1 and AQP5 in contrast to disease control. It was inferenced from findings that both extract of QL exhibited notable antiasthmatic potential might be due to presence of Daidzein-glucuronic acid, 3-Hydroxyphloretin 6'-hexoside, Catechin, Quercetin, and Kaemferol.
草药为治疗哮喘等疾病提供了一种替代方法，因为与合成药物相比，它们的成本低，不良反应相对较少。绵毛柯的叶子传统上用于治疗哮喘。本研究旨在评估栎树叶（QL）甲醇（QLME）和水提物（QLAE）对卵清蛋白（OVA）诱导的哮喘的抗哮喘活性，并通过高效液相色谱-二极管阵列检测器（HPLC-DAD）对 QL 提取物进行化学表征。动物在第 1 天和第 14 天通过腹腔接种 OVA（i.p），然后在第 27 天和第 29 天进行鼻内挑战。QL 的两种提取物（QLME 和 QLAE）在 600、300 和 150mg/kg 及地塞米松（2mg/kg）水平下，通过口服灌胃给药，从第 15 天到第 26 天连续给药。QL 提取物显著降低（p<0.0001-p<0.05）了血液和 BALF 中的总白细胞和白细胞分类计数以及血清 IgE 水平，与疾病对照组相比。两种提取物和地塞米松均显著提高了超氧化物歧化酶、过氧化氢酶和 GSH 的活性，同时降低了治疗组小鼠丙二醛的水平。与疾病对照组相比，提取物和地塞米松处理导致肿瘤坏死因子-α、白细胞介素-4、-5、-13、-6、-1β 和 NF-κB 的表达显著下调（p<0.0001-p<0.05），而水通道蛋白（AQP）1 和 AQP5 的表达显著上调。从研究结果推断，QL 的两种提取物均表现出显著的抗哮喘潜力，这可能是由于存在大豆苷元-葡萄糖醛酸、3-羟基根皮苷 6'-己糖苷、儿茶素、槲皮素和山奈酚。
