Wang Zhenji, Bai Zhe, Yan Jinghua, Liu Tengteng, Li Yingmei, Xu Jiahui, Meng Xiaoqian, Bi Yuefeng
School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.
Collaborative Innovation Center of New Drug Research and Safety Evaluation, Zhengzhou 450001, China.
Chin Herb Med. 2021 Dec 1;14(1):97-103. doi: 10.1016/j.chmed.2021.11.002. eCollection 2022 Jan.
The purpose of this study is to investigate the anti-diabetic effects of linarin, a flavonoid extracted from (CIF), and its potential mechanisms.
The effects of linarin on cell viability and glucose consumption in HepG2 cells were measured. Meanwhile, monosodium glutamate (MSG) mouse model was constructed to monitor the changes of insulin tolerance, glucose tolerance, triglyceride and cholesterol. The protein expression levels of p-AMPK, p-ACC, PEPCK and p-GS were detected by Western blot.
Linarin could increase the relative glucose consumption of HepG2 cells, improve insulin tolerance and glucose tolerance, and decrease the levels of triglyceride and cholesterol of MSG mice. Simultaneously, the expression levels of p-AMPK and p-ACC in HepG2 cells and the liver tissue of MSG mice were increased, while the expression levels of PEPCK and p-GS were decreased after treatment with linarin.
Insulin resistance could be ameliorated by linarin in type 2 diabetes, and its mechanism may be related to AMPK signaling pathway.
本研究旨在探讨从[具体植物名称]中提取的黄酮类化合物木犀草素（CIF）的抗糖尿病作用及其潜在机制。
检测木犀草素对HepG2细胞活力和葡萄糖消耗的影响。同时，构建谷氨酸钠（MSG）小鼠模型，监测胰岛素耐受性、葡萄糖耐受性、甘油三酯和胆固醇的变化。通过蛋白质免疫印迹法检测p-AMPK、p-ACC、磷酸烯醇式丙酮酸羧激酶（PEPCK）和p-GS的蛋白表达水平。
木犀草素可增加HepG2细胞的相对葡萄糖消耗，改善胰岛素耐受性和葡萄糖耐受性，并降低MSG小鼠的甘油三酯和胆固醇水平。同时，木犀草素处理后，HepG2细胞和MSG小鼠肝脏组织中p-AMPK和p-ACC的表达水平升高，而PEPCK和p-GS的表达水平降低。
木犀草素可改善2型糖尿病中的胰岛素抵抗，其机制可能与AMPK信号通路有关。
