Li Jian-Ling, Han Zheng-Zhou, Chi Lian-Feng, Wei Min, Ye Zi, Wu Man-Ting, Lin Hong, Fan Guang-Hui, Xu Lei, Wei Wei-Feng
Qinghai Academy of Agriculture and Forestry Sciences Xining 810016, China Qinghai Plateau Tree Genetics and Breeding Laboratory Xining 810016, China China Resources Sanjiu Medical & Pharmaceutical Co., Ltd. Shenzhen 518110, China Shenzhen Traditional Chinese Medicine Manufacturing Innovation Center Co., Ltd. Shenzhen 518110, China.
China Resources Sanjiu Medical & Pharmaceutical Co., Ltd. Shenzhen 518110, China Shenzhen Traditional Chinese Medicine Manufacturing Innovation Center Co., Ltd. Shenzhen 518110, China.
Zhongguo Zhong Yao Za Zhi. 2022 Oct;47(19):5217-5223. doi: 10.19540/j.cnki.cjcmm.20220616.102.
This study explored the correlation between color and chemical components of Chrysanthemi Indici Flos(CIF), aiming at providing a reference for its procurement, evaluation, and breeding. Colorimeter and ultra-performance liquid chromatograph(UPLC) were used to determine the color(lightness-shade chromaticity value L*, red-green chromaticity value a, yellow-blue chromati-city value b~) and chemical components(cynaroside, linarin, luteolin, apigenin, and chlorogenic acid) of 84 CIF germplasms, respectively. Diversity analysis, correlation analysis, regression analysis, and cluster analysis were performed. The results showed that the color and chemical components of CIF were diversified. Chlorogenic acid was in significantly positive correlation with L* and b* and significantly negative correlation with a*. Cynaroside and grey relational grade γ_i of chemical components were in significantly po-sitive correlation with b* and L*, respectively, whereas linarin, luteolin, and apigenin had no significant correlation with L, a~, or b*. The 84 CIF germplasms were clustered into 4 clades. In addition, germplasms in clade Ⅲ had higher γ_i and total color value(E*_(ab)) than those in other clades, with the best quality and color, and a germplasm with the highest quality, bright yellow color, and highest content of linarin was screened out in this clade. Thus, CIF with bright yellow color had high content of cymaroside and chlorogenic acid and thereby high quality. In summary, the color can be used to quickly predict the quality of CIF. Our results provided data for the evaluation of CIF quality by color and a reference for its procurement and breeding.
本研究探讨了野菊花颜色与化学成分之间的相关性，旨在为其采购、评价和育种提供参考。采用色差仪和超高效液相色谱仪（UPLC）分别测定了84份野菊花种质的颜色（明度-暗度色度值L*、红-绿色度值a、黄-蓝色度值b~）和化学成分（绿原酸、木犀草素、芹菜素、金圣草黄素和绿原酸）。进行了多样性分析、相关性分析、回归分析和聚类分析。结果表明，野菊花的颜色和化学成分具有多样性。绿原酸与L*和b*呈显著正相关，与a*呈显著负相关。绿原酸糖苷和化学成分的灰色关联度γ_i分别与b*和L*呈显著正相关，而木犀草素、芹菜素和金圣草黄素与L*、a*或b无显著相关性。84份野菊花种质被聚类为4个分支。此外，分支Ⅲ中的种质γ_i和总颜色值（E~_(ab)）高于其他分支，品质和颜色最佳，且在该分支中筛选出了一份品质最高、亮黄色且木犀草素含量最高的种质。因此，亮黄色的野菊花绿原酸糖苷和绿原酸含量高，品质优良。综上所述，颜色可用于快速预测野菊花的品质。我们的研究结果为通过颜色评价野菊花品质提供了数据，并为其采购和育种提供了参考。
