Ma Ying-Xian, Sutcharitchan Chayanis, Li Xiao-Die, Meng Qian, Wang Xin, Ji Shen, Cui Ya-Jun
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Chulalongkorn University, Bangkok 10330, Thailand.
Chin Herb Med. 2020 Oct 16;12(4):367-374. doi: 10.1016/j.chmed.2020.10.001. eCollection 2020 Oct.
As traditional techniques for microscopic identification of Chinese medicines currently lack objective and high-quality reference images, here we developed a systemic procedure to be used in microscopic identification of Chinese medicines, which would lead to more objective, effective and accurate identification process.
(Jixueteng in Chinese) was used as the specimen in the development of such procedure. Jixueteng samples were microscopically examined in bright- and dark-field microscopy. Microscopic images were obtained by regular, EDF, and image stitching techniques.
The microscopic images of the characteristics in pulverized Jixueteng were captured, thanks to EDF imaging and image stitching techniques which allowed the detailed and full sighting of each characteristic to be obtained simultaneously. Different layers in anatomical transverse section, including cork, phelloderm, cortex, phloem, cambium, xylem and pith, were distinctively observed. Moreover, by comparing images of bright- and dark-field microscopy, birefringent and non- birefringent components could readily be distinguished.
With application of the developed procedure, high-definition, panoramic and microscopic images were acquired, which could be used as the reference images for microscopic identification of Chinese medicines.
由于目前中药显微鉴定的传统技术缺乏客观、高质量的参考图像，我们在此开发了一种用于中药显微鉴定的系统程序，该程序将使鉴定过程更加客观、有效和准确。
以鸡血藤为标本开展该程序的开发。对鸡血藤样品进行明场和暗场显微镜检查。通过常规、EDF和图像拼接技术获取显微图像。
借助EDF成像和图像拼接技术，获得了鸡血藤粉末特征的显微图像，可同时详细、全面地观察到各个特征。在横切面上清晰观察到不同的组织层，包括木栓层、栓内层、皮层、韧皮部、形成层、木质部和髓。此外，通过比较明场和暗场显微镜图像，可轻松区分双折射和非双折射成分。
应用所开发的程序可获取高清、全景的显微图像，可作为中药显微鉴定的参考图像。
