Department of Plant Sciences, Lahore College for Women University, Lahore, Pakistan.
Government College University, Faisalabad, Pakistan.
Microsc Res Tech. 2019 Oct;82(10):1660-1667. doi: 10.1002/jemt.23332. Epub 2019 Jul 3.
The use of quality control tool for adulteration of Senna (Cassia aungustifolia) a pharmaceutically very important. They were used for multiple health disorders such as constipation, indigestion, epilepsy, asthma, piles, migraine, and heart problems. Two different species of same family or same genus used commercially in Indo-Pak using the same medicine name Senna. One named as Senna (C. aungustifolia) and its adulterant named as Sickle Senna (Cassia obtusifolia).
These two plants were analyzed using classical microscopic techniques light microscopy and the modern chemotaxonomic traits scanning electron microscopy, fluorescence studies and phytochemical studies.
The C. aungustifolia L. had found to be a perennial herb with trilobed pollen, diacytic, paracytic, and anisocytic stomata having smooth walled epidermal cells, whereas the C. obtusifolia stands out as a perennial shrub with spheroidal and circular pollen and paracytic type of stomata having irregular shaped epidermal cells. The powdered drug of C. aungustifolia is dark grayish green, whereas the powdered drug of C. obtusifolia is light green in color. Investigation and other techniques used in this project provided the basis for the authentication of this species.
使用质量控制工具来鉴定番泻叶（狭叶番泻）的掺假情况非常重要，因为番泻叶在药学上非常重要。它们被用于多种健康问题，如便秘、消化不良、癫痫、哮喘、痔疮、偏头痛和心脏问题。在印度-巴基斯坦，两种不同的同科或同属植物被商业上用于使用相同的药物名称番泻叶。一种名为番泻叶（Cassia angustifolia），其掺杂物名为镰叶番泻叶（Cassia obtusifolia）。
使用经典的显微镜技术（光镜）和现代化学分类学特征（扫描电子显微镜、荧光研究和植物化学研究）对这两种植物进行了分析。
C. angustifolia L. 被发现是一种多年生草本植物，具有三叶状花粉、二细胞型、对细胞型和不等细胞型气孔，具有光滑壁的表皮细胞，而 C. obtusifolia 则是一种多年生灌木，具有球形和圆形花粉和对细胞型气孔，具有不规则形状的表皮细胞。C. angustifolia 的粉末药物呈深灰绿色，而 C. obtusifolia 的粉末药物呈浅绿色。本项目中使用的调查和其他技术为该物种的鉴定提供了依据。
