RPRD1B 在热休克反应中发挥重要作用。
An Important Role for RPRD1B in the Heat Shock Response.
机构信息
Mechanisms of Transcription Laboratory, The Francis Crick Institute, London, United Kingdom.
Bioinformatics and Biostatistics, The Francis Crick Institute, London, United Kingdom.
出版信息
Mol Cell Biol. 2022 Oct 20;42(10):e0017322. doi: 10.1128/mcb.00173-22. Epub 2022 Sep 19.
During the heat shock response (HSR), heat shock factor (HSF1 in mammals) binds to target gene promoters, resulting in increased expression of heat shock proteins that help maintain protein homeostasis and ensure cell survival. Besides HSF1, only a relatively few transcription factors with a specific role in ensuring correctly regulated gene expression during the HSR have been described. Here, we use proteomic and genomic (CRISPR) screening to identify a role for RPRD1B in the response to heat shock. Indeed, cells depleted for RPRD1B are heat shock sensitive and show decreased expression of key heat shock proteins (HSPs). These results add to our understanding of the connection between basic gene expression mechanisms and the HSR.
在热休克反应（HSR）期间，热休克因子（哺乳动物中的 HSF1）与靶基因启动子结合，导致热休克蛋白表达增加，这些蛋白有助于维持蛋白质的内稳态并确保细胞存活。除了 HSF1 之外，只有少数几个转录因子在 HSR 期间具有特定的作用，以确保基因表达的正确调节。在这里，我们使用蛋白质组学和基因组学（CRISPR）筛选来确定 RPRD1B 在热休克反应中的作用。事实上，耗尽 RPRD1B 的细胞对热休克敏感，并且关键热休克蛋白（HSPs）的表达降低。这些结果增加了我们对基本基因表达机制与 HSR 之间联系的理解。
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