病毒嘧啶二聚体-DNA糖基化酶的结构组织、进化及分布

Structural organization, evolution, and distribution of viral pyrimidine dimer-DNA glycosylases.

作者信息

Karmanova Alexandra N, Nikulin Nikita A, Zimin Andrei A

机构信息

Laboratory of Molecular Microbiology, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Federal Research Center, Pushchino, 142290 Russia.

Laboratory of Bacteriophage Biology, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Federal Research Center, 142290 Pushchino, Russia.

出版信息

Biophys Rev. 2022 Jun 18;14(4):923-932. doi: 10.1007/s12551-022-00972-4. eCollection 2022 Aug.

DOI:10.1007/s12551-022-00972-4

PMID:36124272

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9481830/

Abstract

UNLABELLED

DNA glycosylases are DNA repair enzymes capable of removing damaged nitrogenous bases, including those formed as a result of UV irradiation with sunlight (approximately 300-400 нм). DNA glycosylases are common not only among bacteria, archaea, and eukaryotes, but some groups of viruses can also encode them. The best-known viral glycosylase is endonuclease V (DenV, Pdg-T4) of Escherichia virus T4, the main substrate of which is cyclobutane pyrimidine dimers. DenV is isolated separately from other large families of glycosylases; it is quite common in nature and has homologs in a number of other viruses and even bacteria. However, the ways of its origin are poorly understood. The best-known DenV homolog is the glycosylase of Chlorella virus strain, PBCV-1 (Cv-pdg). This review contains the main known data on the structure and mechanism of operation of DenV and its homologs. The issues of biological importance and distribution of the enzyme and its homologs among viruses are considered and supplemented separately.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s12551-022-00972-4.

摘要

未标记

DNA糖基化酶是一类DNA修复酶，能够去除受损的含氮碱基，包括因阳光紫外线照射（约300 - 400纳米）而形成的碱基。DNA糖基化酶不仅在细菌、古细菌和真核生物中普遍存在，一些病毒群体也能编码此类酶。最著名的病毒糖基化酶是大肠杆菌病毒T4的内切核酸酶V（DenV，Pdg - T4），其主要底物是环丁烷嘧啶二聚体。DenV与其他大型糖基化酶家族是分开的；它在自然界中相当常见，并且在许多其他病毒甚至细菌中都有同源物。然而，其起源方式尚不清楚。最著名的DenV同源物是小球藻病毒株PBCV - 1的糖基化酶（Cv - pdg）。本综述包含了关于DenV及其同源物的结构和作用机制的主要已知数据。酶及其同源物在病毒中的生物学重要性和分布问题将分别进行讨论和补充。