Filgueiras Marcos Daniel Gomes, Matos Renata Silva, Barreto Lucas Prado, Mascarin Gabriel Moura, Rizzo Pricila Vetrano, Freitas Fernanda Mara Cunha, de Azevedo Prata Márcia Cristina, Monteiro Caio, Fernandes Éverton Kort Kamp
Programa de Pós-graduação em Ciência Animal, Universidade Federal de Goiás, Goiânia, Brazil.
Embrapa Gado de leite, Juiz de Fora, Brazil.
Pest Manag Sci. 2023 Jan;79(1):216-225. doi: 10.1002/ps.7190. Epub 2022 Oct 7.
The control of ticks is challenged by the resistance of tick populations to chemical acaricides. In this study, we evaluated, under laboratory conditions, the efficacy of Heterorhabditis bacteriophora against Rhipicephalus (Boophilus) microplus engorged females with varying body weights (150, 200, 250, 300 or 350 mg per female) or from eight different geographical populations. We also determined the efficacy of H. bacteriophora for tick control under field conditions.
R. microplus engorged females with varying body weights exposed to 150 juveniles of H. bacteriophora resulted in a high control efficacy (97.5% to 98.4%). Tests with females from different geographical populations comprised eight tick strains treated with H. bacteriophora and their respective control groups. The biological parameters of females exposed to nematode treatments did not differ significantly and resulted in 89% to 99% of control efficacy. Trials conducted under field conditions were performed in field plots with Megathyrsus maximus grass. Treatment groups received eight cadavers of Tenebrio molitor fully colonized with H. bacteriophora at 1 week prior to the release of female ticks, whereas control groups were untreated. On the first day of the experiment, six engorged females were distributed in each plot. On day 42 and day 63, the apical portion of the grasses with R. microplus larvae were collected and quantified. The population of R. microplus larvae was reduced up to 73.1% in plots treated with H. bacteriophora at day 63 after treatment.
R. microplus engorged females with varying body weights or from different geographical populations were highly susceptible to H. bacteriophora. The field test demonstrated the efficacy of H. bacteriophora in reducing R. microplus larvae in infested pastures. © 2022 Society of Chemical Industry.
蜱虫种群对化学杀螨剂产生抗性，给蜱虫控制带来了挑战。在本研究中，我们在实验室条件下评估了嗜菌异小杆线虫对不同体重（每只雌蜱150、200、250、300或350毫克）或来自八个不同地理种群的微小牛蜱饱血雌蜱的防治效果。我们还确定了嗜菌异小杆线虫在田间条件下对蜱虫控制的效果。
接触150只嗜菌异小杆线虫幼虫的不同体重微小牛蜱饱血雌蜱，防治效果很高（97.5%至98.4%）。对来自不同地理种群的雌蜱进行的试验包括用嗜菌异小杆线虫处理的八个蜱虫品系及其各自的对照组。接受线虫处理的雌蜱的生物学参数没有显著差异，防治效果为89%至99%。在田间条件下进行的试验在种植巨菌草的田间地块中进行。处理组在释放雌蜱前1周接受八具完全被嗜菌异小杆线虫定殖的黄粉虫尸体，而对照组不进行处理。在实验的第一天，在每个地块中分布六只饱血雌蜱。在第42天和第63天，收集并量化带有微小牛蜱幼虫的草的顶部部分。处理后第63天，用嗜菌异小杆线虫处理的地块中微小牛蜱幼虫的数量减少了73.1%。
不同体重或来自不同地理种群的微小牛蜱饱血雌蜱对嗜菌异小杆线虫高度敏感。田间试验证明了嗜菌异小杆线虫在减少受侵染牧场中微小牛蜱幼虫数量方面的效果。© 2022化学工业协会。
