Facultad de Estudios Superiores Iztacala, Laboratorio de Gametos y Desarrollo Tecnológico, UNAM, Estado de México, Mexico.
Facultad de Estudios Superiores Zaragoza, Laboratorio de Pubertad, Unidad de Investigación en Biología de la Reproducción, UNAM, Ciudad de México, Mexico.
Syst Biol Reprod Med. 2022 Oct-Dec;68(5-6):396-406. doi: 10.1080/19396368.2022.2116369. Epub 2022 Sep 21.
Serotonin is a neurotransmitter that affects the secretion of gonadotropins and testosterone. In prepubertal male rats, serotonin has a stimulating role in testosterone secretion. Here, we used prepubertal male rats to study the effects of -chloroamphetamine (pCA) on circulating testosterone and gonadotropins and markers of apoptosis in germ cells from day 1 to day 5 post-treatment. The intraperitoneal administration of pCA induced a significant reduction in concentrations of hypothalamic serotonin and circulating testosterone, but gonadotropins were not affected. In the seminiferous epithelium of pCA-treated rats, increased the number of germ cells positive to markers of apoptosis, concomitantly with alterations in morphometry and the presence of multinucleated germ cells. Levels of testosterone were reduced starting from 1 day after pCA was administered. The time window between the administration of the pCA and collection of samples was sufficient to detect changes in testosterone levels, in contrast with a previous work where no changes were found. There was a possible relationship between the reduction of testosterone and an increase in the number of germ cells positive to apoptosis markers. However, the mechanism that links pCA-testosterone-germ cell positive to markers of apoptosis is unknown. Our outcomes support the view that pCA exposure during the prepubertal stage has an acute impact on testosterone levels and affects the structure and physiology of seminiferous epithelium.
血清素是一种影响促性腺激素和睾酮分泌的神经递质。在青春期前的雄性大鼠中，血清素对睾酮的分泌有刺激作用。在这里，我们使用青春期前的雄性大鼠研究了 - 氯苯丙胺（pCA）对治疗后第 1 天至第 5 天循环睾酮和促性腺激素以及生殖细胞凋亡标志物的影响。pCA 的腹腔给药导致下丘脑血清素和循环睾酮浓度显着降低，但促性腺激素不受影响。在 pCA 处理大鼠的生精上皮中，凋亡标志物阳性的生殖细胞数量增加，同时形态发生改变和出现多核生殖细胞。pCA 给药后 1 天开始降低睾酮水平。pCA 的给药和样本采集之间的时间窗口足以检测到睾酮水平的变化，这与之前没有发现变化的一项工作形成对比。睾酮的减少与凋亡标志物阳性的生殖细胞数量增加之间可能存在关系。然而，将 pCA-睾酮-生殖细胞与凋亡标志物联系起来的机制尚不清楚。我们的研究结果支持这样一种观点，即青春期前阶段暴露于 pCA 对睾酮水平有急性影响，并影响生精上皮的结构和生理学。
