Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, 100 Dongshan Road, Huaibei 235000, China.
Institute of Plant Protection, Suzhou Academy of Agricultural Sciences, Suzhou 234000, China.
J Agric Food Chem. 2022 Oct 5;70(39):12372-12382. doi: 10.1021/acs.jafc.2c04335. Epub 2022 Sep 21.
Usually, the recognition of sex pheromone signals is restricted to adult moths. Here, our behavioral assay showed that fourth-instar larvae are attracted to cabbage laced with minor sex pheromones Z9,E12-tetradecadienyl acetate (Z9,E12-14:Ac) or Z9-tetradecenyl acetate (Z9-14:Ac). Seven odorant-binding proteins (OBPs) were upregulated after exposure to Z9,E12-14:Ac, and one OBP was upregulated after exposure to Z9-14:Ac. Fluorescence competitive binding assays showed that GOBP2 and OBP7 bound to sex pheromones. RNAi treatment significantly downregulated GOBP2 and OBP7 mRNA expression by 70.37 and 63.27%, respectively. The siOBP-treated larvae were not attracted to Z9,E12-14:Ac or Z9-14:Ac, and the corresponding preference indices were significantly lower than those in siGFP-treated larvae. Therefore, we concluded that GOBP2 and OBP7 are involved in the attraction of larvae to food containing Z9,E12-14:Ac and Z9-14:Ac. These results provide an important basis for exploring the olfactory mechanisms underlying sex pheromone attraction in moth larvae.
通常情况下，性信息素信号的识别仅限于成年飞蛾。在这里，我们的行为测定表明，四龄幼虫被包埋有少量性信息素 Z9、E12-十四碳二烯基乙酸酯 (Z9、E12-14:Ac) 或 Z9-十四碳烯基乙酸酯 (Z9-14:Ac) 的甘蓝吸引。暴露于 Z9、E12-14:Ac 后，有 7 种气味结合蛋白 (OBPs) 上调，暴露于 Z9-14:Ac 后有 1 种 OBP 上调。荧光竞争结合测定表明 GOBP2 和 OBP7 与性信息素结合。RNAi 处理使 GOBP2 和 OBP7 mRNA 表达分别显著下调 70.37%和 63.27%。经 siOBP 处理的幼虫对 Z9、E12-14:Ac 或 Z9-14:Ac 没有吸引力，相应的偏好指数明显低于 siGFP 处理的幼虫。因此，我们得出结论，GOBP2 和 OBP7 参与了幼虫对含有 Z9、E12-14:Ac 和 Z9-14:Ac 的食物的吸引。这些结果为探索飞蛾幼虫性信息素吸引的嗅觉机制提供了重要依据。
