Zhao Qinglong, Wang Xinning, Mustafa Ahsan, Wang Ying, Wang Hongfang, Chi Xuepeng, Xu Baohua, Liu Zhenguo
Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China.
Biomolecules. 2025 Jan 6;15(1):66. doi: 10.3390/biom15010066.
Olfaction mediated by the antennae is a vital sensory modality for arthropods and could be applied as a tool in pest control. The ectoparasitic mite poses a significant threat to the health of the honey bee worldwide and has garnered global attention. To better understand the chemical ecology of this host-parasite relationship, we collected and characterized the volatile organic compounds (VOCs) from and used electroantennography (EAG) to record the responses of honey bee ( and ) antennae to the different VOCs. Fifteen VOCs were detected from using gas chromatography-mass spectrometry (GC-MS), which mainly contained ethyl palmitate, followed by isoamyl alcohol, nonanal, and ethyl oleate. The EAGs for ethyl palmitate were higher at the lowest stimulus loading (5 μg/μL in liquid paraffin) in compared to , suggesting that may have acute sensitivity to low concentrations of some VOCs from . After exposure to ethyl palmitate for 1 h, the relative expression levels of and in significantly increased, as well as the level of in , while showed no significant changes. The results indicate that the EAG response was influenced by the VOC composition and concentration. tended to be more responsive than to the VOCs of . Our findings offer a deeper understanding of how bees recognize , potentially using ethyl palmitate as a chemical cue.
由触角介导的嗅觉是节肢动物至关重要的一种感觉方式，并且可作为害虫防治的一种工具。体外寄生螨对全球范围内蜜蜂的健康构成重大威胁，已引起全球关注。为了更好地理解这种宿主 - 寄生虫关系的化学生态学，我们收集并表征了[具体来源未明确]的挥发性有机化合物（VOCs），并使用触角电图（EAG）记录蜜蜂（[具体品种未明确]和[具体品种未明确]）触角对不同VOCs的反应。使用气相色谱 - 质谱联用仪（GC - MS）从[具体来源未明确]中检测到15种VOCs，主要包含棕榈酸乙酯，其次是异戊醇、壬醛和油酸乙酯。与[具体品种未明确]相比，[具体品种未明确]对棕榈酸乙酯在最低刺激负载量（液体石蜡中5μg/μL）时的EAG值更高，这表明[具体品种未明确]可能对来自[具体来源未明确]的某些低浓度VOCs具有急性敏感性。在暴露于棕榈酸乙酯1小时后，[具体品种未明确]中[相关基因未明确]和[相关基因未明确]的相对表达水平显著增加，[具体品种未明确]中[相关基因未明确]的水平也显著增加，而[相关基因未明确]无显著变化。结果表明EAG反应受VOC组成和浓度的影响。[具体品种未明确]对[具体来源未明确]的VOCs往往比[具体品种未明确]反应更灵敏。我们的研究结果为蜜蜂如何识别[具体来源未明确]提供了更深入的理解，可能是利用棕榈酸乙酯作为一种化学线索。
