Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Seoul 34141, Republic of Korea.
Mol Biol Cell. 2022 Nov 1;33(13):ar129. doi: 10.1091/mbc.E22-03-0097. Epub 2022 Sep 21.
In tissue development and regeneration, the establishment of sharp boundaries between heterotypic cells is essential for the differentiation of tissue functions. During the dynamic rearrangements of constituent cells that result from cell division and collective migration, the segregation boundary encounters various challenges. Several studies have suggested that cortical actomyosin structures play a crucial role in the maintenance of the boundary interface of segregated cell populations, implicating actin-mediated stresses. Examining physical cellular properties such as motility, traction, and intercellular stress, we investigated the formation and maintenance of the stable segregation between epithelial and mesenchymal cell populations devoid of heterotypic adhesions. At the contact boundary, the homotypic adhesion-mediated epithelial aggregates exerted collision-mediated compression against the surrounding mesenchymal cells. Our results demonstrated that heterotypic cell populations established a robust interfacial boundary by accumulating stress from active collisions and repulsions between two dissimilar cell types. Furthermore, the moment of the heterotypic collisions was identified by the existence of a sharp rise in maximum shear stress within the cell cluster.
在组织发育和再生过程中，同种异型细胞之间建立明显的边界对于组织功能的分化至关重要。在细胞分裂和集体迁移导致的组成细胞的动态重排过程中，隔离边界会遇到各种挑战。有几项研究表明，皮质肌动球蛋白结构在维持隔离细胞群体的边界界面方面起着关键作用，这暗示了肌动蛋白介导的应力。通过研究物理细胞特性，如运动性、牵引力和细胞间应力，我们研究了在没有异质黏附的情况下，上皮细胞和间充质细胞群体之间稳定隔离的形成和维持。在接触边界处，同种型黏附介导的上皮聚集物对周围的间充质细胞施加碰撞介导的压缩。我们的结果表明，两种不同类型的细胞之间的主动碰撞和排斥会积累应力，从而使异型细胞群体建立了强大的界面边界。此外，通过细胞簇内最大剪切应力的急剧上升，可以确定异型碰撞的时刻。
