Department of Poultry Science, University of Georgia, Athens 30602, Georgia.
Departments of Population Health and Poultry Science, Poultry Diagnostic and Research Center, Athens, 30602, Georgia.
Poult Sci. 2022 Nov;101(11):102124. doi: 10.1016/j.psj.2022.102124. Epub 2022 Aug 7.
A study was conducted to evaluate effects of phytase and coccidial vaccine on growth performance, bone mineralization, nutrient digestibility, and intestinal gene expression of broiler chickens. The experiment was conducted in a 2 × 4 completely randomized factorial arrangement with 6 replicates per treatment and 10 birds each. Applications of coccidiosis vaccine and different dietary treatments were the 2 main factors in the current study. The dietary treatments included 1) a positive control (PC; 0.90% Ca and 0.45% available P: avP); 2) a negative control (NC; 0.75% Ca and 0.30% AvP); 3) NC + 500 FTU/kg of phytase (NC + 500PHY); and 4) NC + 1500 FTU/kg of phytase (NC + 1500PHY). Data were analyzed using SAS by 2-way ANOVA via GLM procedure. The statistical significance was set at P ≤ 0.05, and means were further separated using Tukey's Test. The results indicated that vaccination had no effect on growth performance except for feed intake from 0 to 14 d but negatively (P < 0.05) regulated bone ash and Ca digestibility. Birds fed with the Ca and P-reduced diet (NC) showed a lower BWG and bone ash compared to birds fed with the normal diet (PC), but supplementing phytase mitigated the negative effects on those birds. Broilers fed the NC diet had higher (P < 0.05) total Ca and P digestibility, and phytate degradation; supplementing phytase further increased P digestibility and phytate degradation of the broilers. A significant interaction (P < 0.05) between phytase and vaccination was observed, suggesting the vaccinated birds fed the PC diet and the unvaccinated birds fed the NC + 1500PHY increased calcium-sensing receptor gene expression compared with the unvaccinated birds fed the PC diet. In conclusion, in spite of coccidiosis vaccine, supplementing phytase at 1,500 FTU/kg alleviated the negative effects on growth performance, bone mineralization, and apparent ileal digestibility of P and phytate.
一项研究评估了植酸酶和球虫疫苗对肉鸡生长性能、骨骼矿化、养分消化率和肠道基因表达的影响。该试验采用 2×4 完全随机因子设计，每个处理设 6 个重复，每个重复 10 只鸡。本研究的 2 个主要因素是球虫疫苗的应用和不同的日粮处理。日粮处理包括 1）阳性对照组（PC；0.90%钙和 0.45%有效磷：avP）；2）阴性对照组（NC；0.75%钙和 0.30%AvP）；3）NC+500FTU/kg 植酸酶（NC+500PHY）；和 4）NC+1500FTU/kg 植酸酶（NC+1500PHY）。数据采用 SAS 通过 GLM 程序进行 2 因素方差分析。统计显著性设置为 P≤0.05，并用 Tukey 检验进一步分离均值。结果表明，除了 0 至 14 日龄的采食量外，疫苗接种对生长性能没有影响，但对骨骼灰分和钙消化率有负向（P＜0.05）调节作用。饲喂低钙和低磷日粮（NC）的鸡比饲喂正常日粮（PC）的鸡体重增长和骨骼灰分更低，但添加植酸酶减轻了对这些鸡的负面影响。饲喂 NC 日粮的肉鸡总钙和磷消化率以及植酸盐降解率较高（P＜0.05），添加植酸酶进一步提高了肉鸡的磷消化率和植酸盐降解率。植酸酶和疫苗接种之间存在显著的相互作用（P＜0.05），表明接种疫苗的鸡饲喂 PC 日粮和未接种疫苗的鸡饲喂 NC+1500PHY 日粮时，与未接种疫苗的鸡饲喂 PC 日粮相比，钙敏感受体基因表达增加。总之，尽管有球虫疫苗，但添加 1500FTU/kg 的植酸酶缓解了生长性能、骨骼矿化和植酸磷表观回肠消化率的负面影响。
