de Almeida Lara Ribeiro, de Souza Joaquim Jéssica, Botelho Lucas Moreira, Vidigal Teofania Heloisa Dutra Amorim, Ecco Roselene, de Souza Trindade Giliane, Paglia Adriano Pereira, Pereira Cíntia Aparecida de Jesus, Dos Santos Lima Walter
Laboratório de Helmintologia Veterinária, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais - UFMG, Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG, 31270-901, Brazil.
Laboratório de Malacologia, Departamento de Zoologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais - UFMG, Belo Horizonte, MG, Brazil.
Parasitol Res. 2023 Jan;122(1):347-352. doi: 10.1007/s00436-022-07656-8. Epub 2022 Sep 22.
In this study, rodents (Rattus rattus) and mollusks (Achatina fulica) were captured in a small forest located in a large metropolitan city in Brazil, and they were examined to investigate possible parasitism by Angiostrongylus cantonensis. The parasites were recovered as helminths from the pulmonary arteries of the synanthropic rodents and as third-stage larvae (with Metastrongylidae family characteristics) from the mollusks. To confirm the species, these larvae were used to experimentally infect Rattus norvegicus for the posterior recovery of adult helminths. To identify the adult helminths, morphological, morphometric, molecular, and phylogenetic techniques were employed. Furthermore, we also characterized the histological lesions associated with parasitism in naturally infected definitive hosts. Our results demonstrated the occurrence of a natural life cycle of A. cantonensis (with the presence of adult helminths) in definitive hosts, Rattus rattus, and third-stage larvae in an intermediate host, A. fulica. In free-living rodents, lesions of granulomatous pneumonia in the lungs and meningitis in the brain were also found. These results warn of the risk of accidental transmission of A. cantonensis to human residents around the park because of the extensive interaction among the fauna of the park, domestic animals, and the surrounding human population.
在本研究中，在巴西一个大都市的一片小森林中捕获了啮齿动物（褐家鼠）和软体动物（非洲大蜗牛），并对它们进行检查，以调查广州管圆线虫的潜在寄生情况。从共生啮齿动物的肺动脉中作为蠕虫回收了寄生虫，从软体动物中作为具有后圆线虫科特征的第三期幼虫回收了寄生虫。为了确认物种，这些幼虫被用于实验性感染挪威大鼠，以便随后回收成虫蠕虫。为了鉴定成虫蠕虫，采用了形态学、形态测量学、分子和系统发育技术。此外，我们还对自然感染终末宿主中与寄生相关的组织学病变进行了特征描述。我们的结果表明，在终末宿主褐家鼠中存在广州管圆线虫的自然生命周期（有成虫蠕虫），在中间宿主非洲大蜗牛中存在第三期幼虫。在自由生活的啮齿动物中，还发现了肺部的肉芽肿性肺炎病变和脑部的脑膜炎病变。这些结果警示，由于公园动物群、家畜和周边人群之间的广泛互动，广州管圆线虫有意外传播给公园周边人类居民的风险。
