Réservoir et Diversité des Pathogènes, Unité Transmission, Institut Pasteur de Guadeloupe, Pointe-à-Pitre, France.
Institute for Advanced Biosciences, Team Host-Pathogen Interactions and Immunity to Infection, INSERM U1209-CNRS UMR5309, Université Grenoble Alpes, Grenoble, France.
Parasit Vectors. 2023 Aug 10;16(1):276. doi: 10.1186/s13071-023-05872-4.
Angiostrongylus cantonensis (rat lungworm) is the main pathogen responsible for eosinophilic meningitis in humans. One of its intermediate snail hosts, Achatina fulica, was already present in many countries around the world before it appeared in the West Indies in the late 1980s. In the French territories in the Caribbean and northern South America, the first cases of human neuroangiostrongyliasis were reported in Martinique, Guadeloupe and French Guiana in 2002, 2013 and 2017, respectively. In order to better characterize angiostrongyliasis in Guadeloupe, particularly its geographical origin and route of introduction, we undertook molecular characterization of adult worms of Angiostrongylus cantonensis and its intermediate host Achatina fulica.
Genomic DNA of adult Angiostrongylus cantonensis and Achatina fulica was extracted and amplified by polymerase chain reaction (PCR) targeting the mitochondrial genes cytochrome B and C for A. cantonensis and 16S ribosomal RNA for A. fulica. The PCR products were sequenced and studied by phylogenetic analysis.
Cytochrome B and cytochrome C molecular markers indicate a monophyletic lineage of A. cantonensis adult worms in Guadeloupe. Two sequences of A. fulica were identified.
These results confirm the recent introduction of both Angiostrongylus cantonensis and Achatina fulica into Guadeloupe. Achatina fulica in Guadeloupe shares a common origin with those in Barbados and New Caledonia, while Angiostrongylus cantonensis in Guadeloupe shares a common origin with those in Brazil, Hawaii and Japan.
广州管圆线虫（鼠肺线虫）是导致人类嗜酸性脑膜炎的主要病原体。它的一种中间宿主非洲大蜗牛，在 20 世纪 80 年代末出现在西印度群岛之前，已经在世界许多国家存在。在加勒比海和南美洲北部的法属领土上，2002 年、2013 年和 2017 年，分别在马提尼克岛、瓜德罗普岛和法属圭亚那首次报告了人类神经血管圆线虫病病例。为了更好地描述瓜德罗普岛的血管圆线虫病，特别是其地理起源和传入途径，我们对广州管圆线虫成虫及其中间宿主非洲大蜗牛进行了分子特征描述。
从广州管圆线虫成虫和非洲大蜗牛中提取基因组 DNA，通过聚合酶链反应（PCR）对线粒体基因细胞色素 B 和 C 进行扩增，用于鉴定广州管圆线虫，对 16S 核糖体 RNA 进行扩增，用于鉴定非洲大蜗牛。对 PCR 产物进行测序，并进行系统发育分析。
细胞色素 B 和细胞色素 C 分子标记表明，瓜德罗普岛的广州管圆线虫成虫具有单系性。鉴定出两种非洲大蜗牛序列。
这些结果证实了广州管圆线虫和非洲大蜗牛最近都被引入了瓜德罗普岛。瓜德罗普岛的非洲大蜗牛与巴巴多斯和新喀里多尼亚的非洲大蜗牛具有共同的起源，而瓜德罗普岛的广州管圆线虫与巴西、夏威夷和日本的广州管圆线虫具有共同的起源。
