使用全组织免疫染色和组织透明化技术在体外器官型小鼠脑切片中测定神经胶质瘤细胞的侵袭和增殖。

Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing.

机构信息

Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, University Utrecht, Utrecht, The Netherlands.

出版信息

STAR Protoc. 2022 Dec 16;3(4):101703. doi: 10.1016/j.xpro.2022.101703. Epub 2022 Sep 21.

DOI:10.1016/j.xpro.2022.101703

PMID:36136755

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9508478/

Abstract

The ex vivo organotypic brain slice invasion model is commonly used to study the growth dynamics of gliomas, primary brain tumors that are known for their invasive behavior. Here, we describe a protocol where the ex vivo organotypic mouse brain slice invasion model is combined with whole-mount immunostaining, tissue clearing, and 3D reconstruction, to visualize and quantify the invasion of glioma cells. In addition, we describe an approach to determine the proliferation rate of the cells within this model. For complete details on the use and execution of this protocol, please refer to Uceda-Castro et al. (2022).

摘要

离体器官型脑切片侵袭模型常用于研究脑胶质瘤的生长动态，脑胶质瘤是一种以侵袭性行为而闻名的原发性脑肿瘤。在这里，我们描述了一种将离体器官型小鼠脑切片侵袭模型与全组织免疫染色、组织透明化和 3D 重建相结合的方法，以可视化和量化脑胶质瘤细胞的侵袭。此外，我们还描述了一种确定该模型中细胞增殖率的方法。有关该方案使用和执行的完整详细信息，请参阅 Uceda-Castro 等人（2022 年）。