Faculty of Veterinary Medicine, Department of Clinical Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas.
Vet Med Sci. 2022 Nov;8(6):2360-2366. doi: 10.1002/vms3.943. Epub 2022 Sep 22.
Optimal amount of vitamin D for the proper functioning of the immune system is different from the required vitamin D amount for bones to prevent rickets. However, reports on vitamin D reference values in dogs are minimal, and there is still not enough information regarding the relationship between vitamin D and various variables such as disease, age, breed, diet type, and so on, as well as its relationship with haematological and serum biochemical parameters.
The present study aimed to determine reference values of 25(OH) Vit D in dogs and its concentration in different groups, categorized based on age, sex, breed, housing conditions, and diet, as well as 25(OH) Vit D relationship with hematology and serum biochemistry parameters.
In this study, 90 healthy dogs were selected to determine the reference value of 25 (OH) Vit D of serum after evaluating of their haematological and biochemical parameters to assess their general health. Dogs were divided into different groups according to above-mentioned variables. Serum 25 (OH) Vit D was subsequently measured by the ELISA method.
The median concentration of 25 (OH) Vit D was 52.50 ng/ml with minimum and maximum amounts of 14.00 and 155.57 ng/ml, respectively. No significant difference was observed between 25 (OH) Vit D levels in the studied dogs regarding their different age, sex, breed, diet, housing condition, and reproductive status. Serum 25 (OH) Vit D concentration is directly correlated with the number of band neutrophils (p < 0.05). We also witnessed indirect correlations between serum 25 (OH) Vit D levels and the number of blood eosinophils and serum glucose (p < 0.05).
In the present study age, sex, breed, housing condition and age had no significant effects on the amounts of 25(OH) vitamin D. According to correlations of vitamin D with MCH, band and eosinophil numbers and glucose, vitamin D may have a role in erythropoiesis and leukocytes response and also in energy metabolism in dog.
对于免疫系统的正常运作，最佳的维生素 D 量不同于预防佝偻病所需的维生素 D 量。然而，关于狗的维生素 D 参考值的报告很少，并且关于维生素 D 与各种变量（如疾病、年龄、品种、饮食类型等）之间的关系及其与血液学和血清生化参数的关系，仍然没有足够的信息。
本研究旨在确定狗的 25（OH）维生素 D 的参考值及其在不同组别的浓度，这些组别的分类依据是年龄、性别、品种、饲养条件和饮食，以及 25（OH）维生素 D 与血液学和血清生化参数的关系。
在这项研究中，选择了 90 只健康的狗，在评估它们的血液学和生化参数后，确定血清 25（OH）维生素 D 的参考值，以评估它们的整体健康状况。狗根据上述变量分为不同的组。随后通过 ELISA 法测量血清 25（OH）维生素 D。
25（OH）维生素 D 的中位数浓度为 52.50ng/ml，最低和最高值分别为 14.00 和 155.57ng/ml。在所研究的狗中，25（OH）维生素 D 水平在不同的年龄、性别、品种、饮食、饲养条件和生殖状态方面没有显著差异。血清 25（OH）维生素 D 浓度与带核中性粒细胞的数量呈直接相关（p<0.05）。我们还观察到血清 25（OH）维生素 D 水平与血液嗜酸性粒细胞和血清葡萄糖的数量之间存在间接相关性（p<0.05）。
在本研究中，年龄、性别、品种、饲养条件和年龄对 25（OH）维生素 D 的含量没有显著影响。根据维生素 D 与 MCH、带核和嗜酸性粒细胞数量以及葡萄糖的相关性，维生素 D 可能在红细胞生成和白细胞反应以及犬的能量代谢中发挥作用。
