Amirnezhad S, Ahmadi Sheikhsarmast S, Khoshnegah J, Mohri M
Graduated from Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
DVSc Student, Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Iran J Vet Res. 2023;24(3):265-269. doi: 10.22099/IJVR.2023.46793.6721.
Optimal vitamin D levels for an effective role in immune function and rickets prevention are thought to vary, but have not yet been definitively determined. Reports on reference values of 25-hydroxyvitamin D (25(OH)D) in cats are limited.
The study provides information about serum 25(OH)D values in cats with different age, gender, breed, diet type, reproductive status, housing condition, and also the relationship between these levels and various hematological and biochemical parameters.
Clinically healthy cats (88) were included in the study. Physical examination and assessment of hematological and biochemical parameters were performed on cats in order to confirm their health status. Reference value of serum 25(OH)D was assayed by ELISA method and the effects of age (under six months and above six months), gender, breed, diet (only commercial diet, only homemade food, mixture of commercial and homemade food), reproduction status, and housing conditions on serum 25(OH)D was determined.
The median, 2.5% and 97.5% of 25(OH)D in sampled cats were 19.74 ng/ml, 3.12 ng/ml, and 92.1 ng/ml, respectively. Serum 25(OH)D concentration was lower when homemade diet was used compared to commercial and mixed diets as well as in cats under six months of age compared to older cats.
Diet type and age can affect serum 25(OH)D levels in healthy cats while other parameters had no significant effects.
人们认为，对免疫功能和预防佝偻病发挥有效作用的最佳维生素D水平有所不同，但尚未最终确定。关于猫血清25-羟维生素D（25(OH)D）参考值的报告有限。
本研究提供了不同年龄、性别、品种、饮食类型、生殖状态、饲养条件的猫血清25(OH)D值的相关信息，以及这些水平与各种血液学和生化参数之间的关系。
本研究纳入了88只临床健康的猫。对猫进行体格检查以及血液学和生化参数评估，以确认其健康状况。采用酶联免疫吸附测定法（ELISA）检测血清25(OH)D的参考值，并确定年龄（6个月以下和6个月以上）、性别、品种、饮食（仅商业饮食、仅自制食物、商业和自制食物混合）、生殖状态和饲养条件对血清25(OH)D的影响。
采样猫血清25(OH)D的中位数、第2.5百分位数和第97.5百分位数分别为19.74 ng/ml、3.12 ng/ml和92.1 ng/ml。与商业饮食和混合饮食相比，采用自制饮食的猫血清25(OH)D浓度较低；与年龄较大的猫相比，6个月以下的猫血清25(OH)D浓度较低。
饮食类型和年龄可影响健康猫的血清25(OH)D水平，而其他参数无显著影响。
