Daneshpour Pardis, Derakhshandeh Nooshin, Nazifi Saeed, Haghighi Zahra Safarzadeh
Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Hematology Laboratory, Shahid Dastgheib Hospital, Shiraz, Iran.
Vet Med Sci. 2025 Sep;11(5):e70530. doi: 10.1002/vms3.70530.
Vitamin D has well-documented antithrombotic effects on coagulation system components, and platelet-associated vitamin D receptors (VDR) play an important role. Adenosine 5'-diphosphate (ADP) induced platelet activation, and thrombin induced a change in the VDR shape, allowing it to bind to fibrinogen and stimulate the aggregation cascade. The effect of vitamin D supplementation on thrombosis in dogs has not been investigated. Eight mixed-breed adult male dogs were selected, and oral vitamin D3 at 50 IU/kg was administered for 42 days. Serum levels of 25-hydroxyvitamin D concentration, platelet number, mean platelet volume (MPV), fibrinogen, prothrombin time (PT) and partial thrombin time were measured before treatment (Day 0) and on Days 14, 28 and 42. The results identified a significant reduction in platelet count following the administration of oral vitamin D (p < 0.0001). There was no significant change in ADP-induced platelet aggregation, MPV, fibrinogen, PT and partial thromboplastin time (PTT). The results suggest that oral administration of vitamin D daily at a dose of 50 IU/kg BW for 42 days leads to a reduction in platelet count; larger studies are necessary to confirm the results and to elucidate potential clinical implications.
维生素D对凝血系统成分具有充分记录的抗血栓形成作用，血小板相关维生素D受体（VDR）发挥着重要作用。二磷酸腺苷（ADP）诱导血小板活化，凝血酶诱导VDR形状改变，使其能够与纤维蛋白原结合并刺激聚集级联反应。维生素D补充剂对犬血栓形成的影响尚未得到研究。选取8只成年雄性杂种犬，口服50 IU/kg的维生素D3，持续给药42天。在治疗前（第0天）以及第14、28和42天测量血清25-羟基维生素D浓度、血小板数量、平均血小板体积（MPV）、纤维蛋白原、凝血酶原时间（PT）和部分凝血活酶时间。结果显示，口服维生素D后血小板计数显著降低（p < 0.0001）。ADP诱导的血小板聚集、MPV、纤维蛋白原、PT和部分凝血活酶时间（PTT）无显著变化。结果表明，以50 IU/kg体重的剂量每日口服维生素D 42天可导致血小板计数降低；需要开展更大规模的研究来证实该结果并阐明潜在的临床意义。
