State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, PR China.
Department of General Surgery, Xiangya Hospital, Central South University, Clinical Research Center for Breast Cancer Control and Prevention in Hunan Province, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, PR China.
Biomater Adv. 2022 Oct;141:213123. doi: 10.1016/j.bioadv.2022.213123. Epub 2022 Sep 15.
Hydrogels, with a three-dimensional network of water-soluble polymer and water, could simulate the critical properties of extracellular matrix, which has been widely used in bone tissue engineering. However, most of conventional hydrogels for bone regeneration are fragile and have poor osteogenic activity, which restricts their applications. In this work, a novel nanoparticle-hydrogel composite consisting of physically cross-linked double-network loaded with MgO-AgO nanocomposites was developed by the sol-gel method. The Mg released from MgO-AgO nanocomposites was used as an ionic cross-linking site of sodium alginate (SA), while the hydrophobic micelles in the polyacrylamide (PAAM) network is acted as another crosslinking point. The results indicated that the novel nanoparticle-hydrogel composites had good self-recovery ability and excellent mechanical properties compared with the conventional sodium alginate (SA)/polyacrylamide (PAAM) hydrogels. Additionally, it showed a slow release of Mg and Ag ions due to the dual function of the embedding effect of hydrogels and the increasing pH of the solution induced by the hydrolysis of sodium alginate. In terms of in vitro tests, the nanoparticle-hydrogel composites showed significantly stimulatory effects on the proliferation and differentiation of SaOS-2 cells. In addition, the antibacterial effects of the nanoparticle-hydrogel composites were gradually enhanced with the increase of MgO-AgO content.
水凝胶是一种由水溶性聚合物和水组成的三维网络，能够模拟细胞外基质的关键特性，因此已被广泛应用于骨组织工程中。然而，大多数用于骨再生的传统水凝胶较为脆弱，且成骨活性较差，这限制了它们的应用。在这项工作中，通过溶胶-凝胶法制备了一种由物理交联的双网络组成的新型纳米颗粒-水凝胶复合材料，该双网络中负载有 MgO-AgO 纳米复合材料。MgO-AgO 纳米复合材料释放的 Mg 用作海藻酸钠（SA）的离子交联位点，而聚丙烯酰胺（PAAM）网络中的疏水胶束则充当另一个交联点。结果表明，与传统的海藻酸钠（SA）/聚丙烯酰胺（PAAM）水凝胶相比，新型纳米颗粒-水凝胶复合材料具有良好的自恢复能力和优异的机械性能。此外，由于水凝胶的嵌入效应以及海藻酸钠水解引起的溶液 pH 值升高的双重作用，该复合材料表现出 Mg 和 Ag 离子的缓慢释放。在体外试验中，纳米颗粒-水凝胶复合材料对 SaOS-2 细胞的增殖和分化表现出显著的促进作用。此外，纳米颗粒-水凝胶复合材料的抗菌效果随着 MgO-AgO 含量的增加而逐渐增强。
