Attia Zeinab R, Zedan Mohamed M, Mutawi Thuraya M, Saad Entsar A, Abd El Azeem Rania A, El Basuni Mohamed A
Mansoura University Children's Hospital, Mansoura University, Mansoura 35516, Egypt.
Department of Pediatrics, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.
Children (Basel). 2022 Aug 24;9(9):1271. doi: 10.3390/children9091271.
Permanent systemic inflammation is a defining feature of systemic lupus erythematosus (SLE), which affects multiple organs. Gelatinase B/matrix metalloproteinase-9 (MMP-9) is an essential protease investigated in inflammation that has been linked to SLE. The study’s objective was to investigate the relationship between the rs3918249 T/C and rs17576 A/G SNPs in the MMP-9 gene with SLE. The study was conducted with 100 SLE cases and 100 age/sex-matched healthy individuals. TaqManTM SNP was used for genotyping by real time PCR on the Artus Rotor-Gene Qiagen equipment. Haplotypes (TG: OR = 0.226, 95% CI = 0.119−0.429) and (CA: OR = 0.36, 95% CI = 0.2206−0.631), both with a p-value < 0.001 were substantially linked to a lower incidence of SLE. Conversely, the risk of SLE was not associated with the individual SNPs studied. The haplotype analysis was more significant than the SNP analysis and may correlate with the decreased risk of SLE in children and adolescents in Egypt.
持续性全身炎症是系统性红斑狼疮（SLE）的一个决定性特征，该病会影响多个器官。明胶酶B/基质金属蛋白酶-9（MMP-9）是一种在炎症中被研究的重要蛋白酶，它与SLE有关。该研究的目的是调查MMP-9基因中rs3918249 T/C和rs17576 A/G单核苷酸多态性（SNP）与SLE之间的关系。该研究对100例SLE患者和100名年龄/性别匹配的健康个体进行。使用TaqManTM SNP通过实时荧光定量PCR在艾本德Rotor-Gene Qiagen仪器上进行基因分型。单倍型（TG：比值比[OR]=0.226，95%置信区间[CI]=0.119−0.429）和（CA：OR=0.36，95%CI=0.2206−0.631），两者p值均<0.001，与SLE较低的发病率显著相关。相反，SLE的风险与所研究的单个SNP无关。单倍型分析比SNP分析更具显著性，可能与埃及儿童和青少年SLE风险降低相关。
