Department of Laboratories, Immunology Lab, Mansoura University Children's Hospital, Mansoura, Egypt.
Department of Pediatrics, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Biomark Med. 2021 Dec;15(17):1669-1680. doi: 10.2217/bmm-2021-0203. Epub 2021 Nov 8.
To demonstrate whether sCD14 and (rs2569190 A/G and rs2569191 C/T) genetic variants are associated with systemic lupus erythematosus (SLE) risk, for the first time, in Egyptian pediatrics and adolescents. sCD14 concentrations were determined in plasma of 95 SLE cases and 98 healthy controls using ELISA assay. Genotyping was performed using TaqMan technology. sCD14 levels were elevated in SLE. Individuals with T, CT and TT genotypes in rs2569191 were of significant risk (odds ratio = 1.471-2.035, 95% CI = 1.138-3.471) and those with combined CT+TT and haplotype GT were of higher risk of SLE (odds ratio = 1.660-1.758, 95% CI = 1.003-3.106, p < 0.05). sCD14 levels and polymorphism were not correlated with SLE clinical and laboratory features. In SLE, sCD14 levels are associated with rs2569190 A/G. Genotype CT+TT in rs2569191 C/T and haplotype GT are associated with SLE risk in Egyptian pediatric and adolescents.
为了首次在埃及儿科和青少年中证明 sCD14 和 （rs2569190 A/G 和 rs2569191 C/T） 遗传变异是否与系统性红斑狼疮（SLE）风险相关，我们使用 ELISA 测定法测定了 95 例 SLE 病例和 98 例健康对照者血浆中的 sCD14 浓度。使用 TaqMan 技术进行基因分型。SLE 患者的 sCD14 水平升高。rs2569191 中 TT 基因型、CT 基因型和 TT 基因型个体具有显著风险（比值比=1.471-2.035，95%可信区间=1.138-3.471），并且具有 CT+TT 基因型和 GT 基因型的个体 SLE 风险更高（比值比=1.660-1.758，95%可信区间=1.003-3.106，p<0.05）。sCD14 水平和多态性与 SLE 的临床和实验室特征无关。在 SLE 中，sCD14 水平与 rs2569190 A/G 相关。rs2569191 C/T 中的 CT+TT 基因型和 GT 基因型与埃及儿科和青少年的 SLE 风险相关。
