Aati Hanan Y, Anwar Mariyam, Al-Qahtani Jawaher, Al-Taweel Areej, Khan Kashif-Ur-Rehman, Aati Sultan, Usman Faisal, Ghalloo Bilal Ahmad, Asif Hafiz Muhammad, Shirazi Jafir Hussain, Abbasi Aliza
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
Antibiotics (Basel). 2022 Aug 26;11(9):1155. doi: 10.3390/antibiotics11091155.
Forssk. (Moraceae family) is an important medicinal plant that has not been previously investigated for its phytochemical and biological potential. Phytochemical screening, total bioactive content, and GCMS analysis were used to determine its phytoconstituents profile. Antioxidant, antibacterial, antifungal, anti-viral, cytotoxicity, thrombolytic, and enzyme inhibition activities were examined for biological evaluation. The plant extract exhibited the maximum total phenolic (89.47 ± 3.21 mg GAE/g) and total flavonoid contents (129.2 ± 4.14 mg QE/g), which may be related to the higher antioxidant potential of the extract. The extract showed strong α-amylase (IC 5 ± 0.21 µg/mL) and α-glucosidase inhibition activity (IC 5 ± 0.32 µg/mL). Significant results were observed in the case of antibacterial, antifungal, and anti-viral activities. The extract inhibited the growth of HepG2 cells in a dose-dependent manner. The GCMS analysis of the extract provided the preliminary identification of 28 phytocompounds. In addition, the compounds identified by GCMS were subjected to in silico molecular docking analysis in order to identify any interactions between the compounds and enzymes (α-amylase and α-glucosidase). After that, the best-docked compounds were subjected to ADMET studies which provide information on pharmacokinetics, drug-likeness, physicochemical properties, and toxicity. The present study highlighted that the ethanol extract of has antidiabetic, antimicrobial, anti-viral, and anti-cancer potentials that can be further explored for novel drug development.
Forssk.（桑科）是一种重要的药用植物，此前尚未对其植物化学和生物学潜力进行研究。采用植物化学筛选、总生物活性成分测定和气相色谱-质谱联用（GCMS）分析来确定其植物成分概况。通过检测抗氧化、抗菌、抗真菌、抗病毒、细胞毒性、溶栓和酶抑制活性进行生物学评价。该植物提取物表现出最高的总酚含量（89.47±3.21毫克没食子酸当量/克）和总黄酮含量（129.2±4.14毫克槲皮素当量/克），这可能与提取物较高的抗氧化潜力有关。该提取物显示出较强的α-淀粉酶抑制活性（IC50±0.21微克/毫升）和α-葡萄糖苷酶抑制活性（IC50±0.32微克/毫升）。在抗菌、抗真菌和抗病毒活性方面观察到显著结果。该提取物以剂量依赖性方式抑制HepG2细胞的生长。提取物的GCMS分析初步鉴定出28种植物化合物。此外，对GCMS鉴定出的化合物进行了计算机模拟分子对接分析，以确定这些化合物与酶（α-淀粉酶和α-葡萄糖苷酶）之间的任何相互作用。之后，对对接效果最佳的化合物进行了药物代谢动力学、药物相似性、理化性质和毒性等方面的ADMET研究。本研究强调，Forssk.的乙醇提取物具有抗糖尿病、抗菌、抗病毒和抗癌潜力，可进一步探索用于新药开发。
