Liang Huixian, Li Xinhui, Yan He
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Department of Microbiology, University of Wisconsin-La Crosse, La Crosse, WI 54601, USA.
Antibiotics (Basel). 2022 Sep 9;11(9):1225. doi: 10.3390/antibiotics11091225.
Multidrug-resistant (MDR) poses a seriously threat to public health. The aim of this study was to better understand the genetic structure of its plasmids and chromosomes. The whole-genome sequence of 200 isolated from the liver of a swine with diarrhea in China was determined using PacBio RS II and Illumina MiSeq sequencing. The complete sequences of the chromosomal DNA and the plasmids were analyzed for the presence of resistance genes. The phylogenetic trees revealed that 200 displayed the closest relationship to a human-associated strain from Thailand. 200 contained two plasmids, pYhe2001 and pYhe2002, belonging to the incompatibility groups IncH-HI1B and IncF-FIA. The plasmid pYhe2001 was a novel plasmid containing four types of heavy metal resistance genes and a novel Tn transposon flanked by two copies of IS at both ends. Mixed plasmids could be transferred from 200 to DH5α through transformation together. This study reported the first time a novel plasmid pYhe2001 from swine origin 200, suggesting that the plasmids may act as reservoirs for various antimicrobial resistance genes and transport multiple resistance genes in of both animal and human origin.
多重耐药（MDR）对公众健康构成严重威胁。本研究的目的是更好地了解其质粒和染色体的遗传结构。使用PacBio RS II和Illumina MiSeq测序确定了从中国一头腹泻猪肝脏中分离出的200株的全基因组序列。分析了染色体DNA和质粒的完整序列中耐药基因的存在情况。系统发育树显示，200株与来自泰国的一株人类相关菌株关系最为密切。200株含有两个质粒，pYhe2001和pYhe2002，分别属于不相容群IncH-HI1B和IncF-FIA。质粒pYhe2001是一种新型质粒，含有四种重金属抗性基因和一个新型Tn转座子，两端各有两个IS拷贝。混合质粒可以通过共转化从200株转移到DH5α中。本研究首次报道了来自猪源200株的新型质粒pYhe2001，表明质粒可能作为各种抗菌抗性基因的储存库，并在动物和人类来源的菌株中传递多种抗性基因。
