State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Green Chemistry and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China.
Biosensors (Basel). 2022 Sep 16;12(9):764. doi: 10.3390/bios12090764.
As an important detection tool in biochemistry, fluorescence detection has wide applications. Quantitative detection can be achieved by detecting fluorescence signals excited by excitation light at a specific wavelength range. Therefore, the key to fluorescence detection is the stable control of the excitation light and the accurate acquisition of weak photoelectric signals. Moreover, to improve portability and instantaneity, devices are developing in miniaturization and integration. As the core of such devices, fluorescence detectors should also have these features. Under this circumstance, we designed a highly integrated and diminutive fluorescence detector and focused on its excitation light driving and photoelectric signal processing. A current-light dual negative feedback light-emitting diode (LED) driving circuit was proposed to obtain constant current and luminance. In addition, a silicon photodiode (PD) was used to receive and convert the fluorescence signal to an electric signal. Then, amplifying, filtering, and analog-to-digital (A/D) converting were applied to make the detection of weak fluorescence signals possible. The test results showed that the designed circuit has wonderful performance, and the detector shows good linearity (R = 0.9967) and sensitivity (LOD = 0.077 nM) in the detection of solution. Finally, a real-time fluorescence polymerase chain reaction (real-time PCR) of was carried out on a homemade platform equipped with this detector, indicating that the detector met the requirements of real-time PCR detection.
作为生物化学中一种重要的检测工具，荧光检测具有广泛的应用。通过检测在特定波长范围内激发光激发的荧光信号，可以实现定量检测。因此，荧光检测的关键在于稳定控制激发光和准确获取微弱光电信号。此外，为了提高便携性和即时性，设备正在向小型化和集成化方向发展。作为此类设备的核心，荧光探测器也应具有这些特点。在这种情况下，我们设计了一种高度集成和微型化的荧光探测器，并专注于其激发光驱动和光电信号处理。提出了一种电流-光双重负反馈发光二极管（LED）驱动电路，以获得恒定的电流和亮度。此外，使用硅光电二极管（PD）接收并将荧光信号转换为电信号。然后，对其进行放大、滤波和模数（A/D）转换，以实现对弱荧光信号的检测。测试结果表明，所设计的电路性能优异，该探测器在检测溶液时具有良好的线性度（R = 0.9967）和灵敏度（LOD = 0.077 nM）。最后，在配备该探测器的自制平台上进行了实时荧光聚合酶链反应（实时 PCR），表明该探测器满足实时 PCR 检测的要求。
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